Sarunic Marinko V, Applegate Brian E, Izatt Joseph A
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.
Opt Lett. 2005 Sep 15;30(18):2391-3. doi: 10.1364/ol.30.002391.
Optical coherence tomography (OCT) provides micrometer-scale structural imaging by coherent detection of backscattered light. Molecular contrast in OCT has been demonstrated using transient absorption, coherent anti-Stokes Raman scattering, and second-harmonic (SH) generation. The sensitivity of molecular contrast signals can be enhanced by use of Fourier domain techniques. We have constructed a spectrometer-based Fourier domain SH-OCT system for simultaneous acquisition of the fundamental and SH signals. We report a >30 dB increase in SH sensitivity over a similar time domain SH-OCT system and demonstrate contrast between cartilage and bone using collagen as the contrast agent.
光学相干断层扫描（OCT）通过对背向散射光的相干检测提供微米级的结构成像。利用瞬态吸收、相干反斯托克斯拉曼散射和二次谐波（SH）产生等技术已在OCT中实现了分子对比度成像。使用傅里叶域技术可以提高分子对比度信号的灵敏度。我们构建了基于光谱仪的傅里叶域SH-OCT系统，用于同时采集基波信号和SH信号。我们报告称，与类似的时域SH-OCT系统相比，SH灵敏度提高了30 dB以上，并使用胶原蛋白作为造影剂展示了软骨和骨骼之间的对比度。
