Wang Ke-Ying
Guizhou Institute for Drug Control, Guiyang 550004, China.
Zhongguo Zhong Yao Za Zhi. 2005 Aug;30(15):1171-2.
To establish the qualitative and quantitative methods of gallic acid in pomegranate rind.
The thin layer chromatographic method was used for identitication, and the high performance liquid chromatographic method was used for assay. Extracts were separated on a Diamonsil C18 column eluted with the mobile phase of a mixture of acetonitrile containing 0.2% methanol and water containing 0.1% phosphoric acid and 0.1% TEA (3:97). The detection wavelength was set at 216 nm, and colum temperature was 40 degrees C.
The calibration curve was linear in the range of 0.085-0.768 microg (r = 0.9996). The average recovery was 96.7% (RSD 0.8%, n = 5). 20 batches of the crude drug were identified and assayed, with the methods.
The methods were sensitive and reliable, and can be used for quality control of the pomegranate rind.
建立石榴皮中没食子酸的定性及定量方法。
采用薄层色谱法进行鉴别，高效液相色谱法进行含量测定。提取物在 Diamonsil C18 柱上分离，以含 0.2%甲醇的乙腈与含 0.1%磷酸和 0.1%三乙胺的水（3:97）的混合液为流动相洗脱。检测波长设定为 216 nm，柱温为 40℃。
校准曲线在 0.085 - 0.768 μg 范围内呈线性（r = 0.9996）。平均回收率为 96.7%（RSD 0.8%，n = 5）。采用该方法对 20 批药材进行了鉴别和含量测定。
该方法灵敏可靠，可用于石榴皮的质量控制。
