[Study on the qualitative and quantitative methods of gallic acid in pomegranate rind].

OBJECTIVE

To establish the qualitative and quantitative methods of gallic acid in pomegranate rind.

METHOD

The thin layer chromatographic method was used for identitication, and the high performance liquid chromatographic method was used for assay. Extracts were separated on a Diamonsil C18 column eluted with the mobile phase of a mixture of acetonitrile containing 0.2% methanol and water containing 0.1% phosphoric acid and 0.1% TEA (3:97). The detection wavelength was set at 216 nm, and colum temperature was 40 degrees C.

RESULT

The calibration curve was linear in the range of 0.085-0.768 microg (r = 0.9996). The average recovery was 96.7% (RSD 0.8%, n = 5). 20 batches of the crude drug were identified and assayed, with the methods.

CONCLUSION

The methods were sensitive and reliable, and can be used for quality control of the pomegranate rind.