Toma Claudia, Lu Yan, Higa Naomi, Nakasone Noboru, Chinen Isabel, Baschkier Ariela, Rivas Marta, Iwanaga Masaaki
Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan.
J Clin Microbiol. 2003 Jun;41(6):2669-71. doi: 10.1128/JCM.41.6.2669-2671.2003.
A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube.
开发了一种用于鉴定人致泻性大肠杆菌的多重聚合酶链反应（PCR）检测方法。为每一类选定的靶标分别是：肠致病性大肠杆菌的eae、产志贺毒素大肠杆菌的stx、肠产毒素大肠杆菌的elt和est、肠侵袭性大肠杆菌的ipaH以及肠聚集性大肠杆菌的aggR。该检测方法可在单个反应管中对致泻性大肠杆菌菌株进行分类。
