用于鉴定人致腹泻性大肠杆菌的多重聚合酶链反应检测法
Multiplex PCR assay for identification of human diarrheagenic Escherichia coli.
作者信息
Toma Claudia, Lu Yan, Higa Naomi, Nakasone Noboru, Chinen Isabel, Baschkier Ariela, Rivas Marta, Iwanaga Masaaki
机构信息
Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan.
出版信息
J Clin Microbiol. 2003 Jun;41(6):2669-71. doi: 10.1128/JCM.41.6.2669-2671.2003.
Abstract
A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube.
摘要
开发了一种用于鉴定人致泻性大肠杆菌的多重聚合酶链反应（PCR）检测方法。为每一类选定的靶标分别是：肠致病性大肠杆菌的eae、产志贺毒素大肠杆菌的stx、肠产毒素大肠杆菌的elt和est、肠侵袭性大肠杆菌的ipaH以及肠聚集性大肠杆菌的aggR。该检测方法可在单个反应管中对致泻性大肠杆菌菌株进行分类。
相似文献
1
Multiplex PCR assay for identification of human diarrheagenic Escherichia coli.用于鉴定人致腹泻性大肠杆菌的多重聚合酶链反应检测法
J Clin Microbiol. 2003 Jun;41(6):2669-71. doi: 10.1128/JCM.41.6.2669-2671.2003.
2
Single multiplex assay to identify simultaneously enteropathogenic, enteroaggregative, enterotoxigenic, enteroinvasive and Shiga toxin-producing Escherichia coli strains in Brazilian children.用于同时鉴定巴西儿童中致病性大肠杆菌、聚集性大肠杆菌、产肠毒素大肠杆菌、侵袭性大肠杆菌和产志贺毒素大肠杆菌菌株的单一多重检测法。
FEMS Microbiol Lett. 2007 Feb;267(2):145-50. doi: 10.1111/j.1574-6968.2006.00580.x.
3
Single multiplex PCR assay to identify simultaneously the six categories of diarrheagenic Escherichia coli associated with enteric infections.用于同时鉴定与肠道感染相关的六类致腹泻性大肠杆菌的单一多重聚合酶链反应检测法。
J Clin Microbiol. 2005 Oct;43(10):5362-5. doi: 10.1128/JCM.43.10.5362-5365.2005.
4
Octaplex PCR and fluorescence-based capillary electrophoresis for identification of human diarrheagenic Escherichia coli and Shigella spp.用于鉴定人致泻性大肠杆菌和志贺氏菌属的八重PCR及荧光毛细管电泳法
J Microbiol Methods. 2007 Feb;68(2):331-41. doi: 10.1016/j.mimet.2006.09.013. Epub 2006 Oct 31.
5
Rapid categorization of pathogenic Escherichia coli by multiplex PCR.通过多重聚合酶链反应对致病性大肠杆菌进行快速分类
Microbiol Immunol. 2005;49(6):485-92. doi: 10.1111/j.1348-0421.2005.tb03752.x.
6
Detection of diarrheagenic Escherichia coli by use of melting-curve analysis and real-time multiplex PCR.利用熔解曲线分析和实时多重聚合酶链反应检测致泻性大肠杆菌
J Clin Microbiol. 2008 May;46(5):1752-7. doi: 10.1128/JCM.02341-07. Epub 2008 Mar 5.
7
Real-time multiplex PCR assay and melting curve analysis for identifying diarrheagenic Escherichia coli.实时多重聚合酶链反应检测和熔解曲线分析鉴定致腹泻性大肠埃希菌。
J Clin Microbiol. 2013 Mar;51(3):1031-3. doi: 10.1128/JCM.02478-12. Epub 2013 Jan 9.
8
A novel single-step multiplex polymerase chain reaction assay for the detection of diarrheagenic Escherichia coli.一种用于检测致泻性大肠杆菌的新型一步多重聚合酶链反应检测法。
J Microbiol Methods. 2013 Mar;92(3):289-92. doi: 10.1016/j.mimet.2012.12.010. Epub 2012 Dec 24.
9
Simple and rapid multiplex PCR for identification of the main human diarrheagenic Escherichia coli.用于鉴定主要致人类腹泻大肠杆菌的简单快速多重 PCR。
Microbiol Res. 2012 Oct 12;167(9):564-70. doi: 10.1016/j.micres.2011.11.006. Epub 2011 Dec 20.
10
Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR.通过使用新型单步多重PCR鉴定表达中等毒力因子谱的非常规肠道致病性大肠杆菌分离株。
Appl Environ Microbiol. 2007 May;73(10):3380-90. doi: 10.1128/AEM.02855-06. Epub 2007 Mar 30.
引用本文的文献
1
Incidence rates of resistant enterotoxigenic Escherichia coli in fresh vegetables and salads.新鲜蔬菜和沙拉中耐抗生素产肠毒素大肠杆菌的发病率
Access Microbiol. 2025 Jul 28;7(7). doi: 10.1099/acmi.0.000957.v3. eCollection 2025.
2
infection risk modeling in Mexico city's flood water.墨西哥城洪水中的感染风险建模
Water Sci Technol. 2022 Apr;85(7):2161-2172. doi: 10.2166/wst.2022.094. Epub 2022 Mar 16.
3
Could Exotic Birds Play a Significant Role in the Emergence of Antibiotic-Resistant Microorganisms?外来鸟类会在抗生素耐药微生物的出现中扮演重要角色吗？
Curr Microbiol. 2025 Feb 28;82(4):161. doi: 10.1007/s00284-025-04134-z.
4
Molecular characterization of common zoonotic protozoan parasites and bacteria causing diarrhea in dairy calves in Ningxia Hui Autonomous Region, China.中国宁夏回族自治区引起犊牛腹泻的常见人畜共患原虫和细菌的分子特征。
Parasite. 2024;31:60. doi: 10.1051/parasite/2024059. Epub 2024 Oct 1.
5
Comparison of metagenomic and traditional methods for diagnosis of enteric infections.宏基因组学与传统方法诊断肠道感染的比较。
mBio. 2024 Apr 10;15(4):e0342223. doi: 10.1128/mbio.03422-23. Epub 2024 Mar 15.
6
Tracing the Evolutionary Pathways of Serogroup O78 Avian Pathogenic .追踪O78血清型禽致病性大肠杆菌的进化途径
Antibiotics (Basel). 2023 Dec 9;12(12):1714. doi: 10.3390/antibiotics12121714.
7
Detection and characterization of potentially hybrid enteroaggregative Escherichia coli (EAEC) strains isolated from urinary tract infection.检测和鉴定从尿路感染分离的潜在混合性肠聚集性大肠杆菌（EAEC）菌株。
Braz J Microbiol. 2024 Mar;55(1):1-9. doi: 10.1007/s42770-023-01195-9. Epub 2023 Dec 1.
8
Resistance and Virulence Surveillance in Isolated from Commercial Meat Samples: A One Health Approach.从商业肉类样本中分离出的[病原体名称未给出]的耐药性和毒力监测：一种一体化健康方法。
Microorganisms. 2023 Nov 6;11(11):2712. doi: 10.3390/microorganisms11112712.
9
Molecular Characterization of spp., , and in Dairy Goat Kids with Diarrhea in Partial Regions of Shaanxi Province, China.中国陕西省部分地区腹泻奶山羊羔羊中 spp.、 、 和 的分子特征分析
Animals (Basel). 2023 Sep 14;13(18):2922. doi: 10.3390/ani13182922.
10
Are non-lactose-fermenting important diarrhoeal pathogens in children and adults?非乳糖发酵菌是儿童和成人重要的腹泻病原体吗？
Access Microbiol. 2023 Jul 12;5(7). doi: 10.1099/acmi.0.000459.v3. eCollection 2023.
本文引用的文献
1
Detection of EspB using reversed passive latex agglutination: application to determination of enteropathogenic Escherichia coli.使用反向被动乳胶凝集法检测EspB：在肠致病性大肠杆菌检测中的应用
Diagn Microbiol Infect Dis. 2002 May;43(1):7-12. doi: 10.1016/s0732-8893(02)00363-2.
2
Comparison of DNA hybridization and PCR assays for detection of putative pathogenic enteroadherent Escherichia coli.用于检测假定致病性肠道黏附性大肠杆菌的DNA杂交和聚合酶链反应检测方法的比较
J Clin Microbiol. 2002 Apr;40(4):1254-8. doi: 10.1128/JCM.40.4.1254-1258.2002.
3
Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa.通过针对stx1、stx2、eae、ehxA和saa的多重PCR直接检测和鉴定产志贺毒素大肠杆菌
J Clin Microbiol. 2002 Jan;40(1):271-4. doi: 10.1128/JCM.40.1.271-274.2002.
4
Multiplex polymerase chain reaction assay for identification of enterotoxigenic Escherichia coli strains.用于鉴定产肠毒素大肠杆菌菌株的多重聚合酶链反应检测法
J Vet Diagn Invest. 2001 Jul;13(4):308-11. doi: 10.1177/104063870101300405.
5
Diversity of surface structures and virulence genetic markers among enteroaggregative Escherichia coli (EAEC) strains with and without the EAEC DNA probe sequence.具有和不具有EAEC DNA探针序列的肠聚集性大肠杆菌（EAEC）菌株之间表面结构和毒力遗传标记的多样性。
FEMS Microbiol Lett. 2001 Jul 24;201(2):163-8. doi: 10.1111/j.1574-6968.2001.tb10751.x.
6
Identification of human enterovirulent Escherichia coli strains by multiplex PCR.通过多重聚合酶链反应鉴定人肠道致病性大肠杆菌菌株
J Clin Lab Anal. 2001;15(2):100-3. doi: 10.1002/jcla.9.
7
8
Multiplex PCRs for identification of Escherichia coli virulence genes.用于鉴定大肠杆菌毒力基因的多重聚合酶链反应
J Clin Microbiol. 2000 May;38(5):2001-4. doi: 10.1128/JCM.38.5.2001-2004.2000.
9
Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new intimin variant.人和动物肠出血性及肠致病性大肠杆菌中紧密黏附素基因的分型：一种新型紧密黏附素变体的特征分析
Infect Immun. 2000 Jan;68(1):64-71. doi: 10.1128/IAI.68.1.64-71.2000.
10
Phylogenetic analysis of enteroaggregative and diffusely adherent Escherichia coli.聚集性和弥漫性黏附性大肠杆菌的系统发育分析
Infect Immun. 1999 Jun;67(6):2692-9. doi: 10.1128/IAI.67.6.2692-2699.1999.
Zotero 插件