Lecso-Bornet M, Pierre J, Sarkis-Karam D, Lubera S, Bergogne-Berezin E
Department of Microbiology, Hôpital X. Bichat-Claude Bernard, Paris, France.
Antimicrob Agents Chemother. 1992 Mar;36(3):669-71. doi: 10.1128/AAC.36.3.669.
The in vitro susceptibilities of 75 clinical isolates of Xanthomonas maltophilia to nalidixic acid, five fluoroquinolones, latamoxef, and doxycycline were determined. Spontaneous mutants were selected, at a frequency of about 10(-5) to 10(-7) from four strains by culturing the strains in the presence of each quinolone. Selection in the presence of nalidixic acid provided mutants that were either resistant only to that compound or that exhibited cross-resistance to all the fluoroquinolones tested. Cross-resistance was always observed for mutants selected on any of the five fluoroquinolones. It was always associated with chloramphenicol resistance and, frequently, with doxycycline resistance. The electrophoretic alterations of the outer membrane proteins of the mutants suggest that different mechanisms may be involved in quinolone resistance in X. maltophilia.
测定了75株嗜麦芽窄食单胞菌临床分离株对萘啶酸、5种氟喹诺酮类药物、拉氧头孢和强力霉素的体外敏感性。通过在每种喹诺酮类药物存在的情况下培养菌株，从4株菌株中以约10^(-5)至10^(-7)的频率选择自发突变体。在萘啶酸存在下进行选择得到的突变体要么仅对该化合物耐药，要么对所有测试的氟喹诺酮类药物表现出交叉耐药性。在任何一种氟喹诺酮类药物上选择的突变体总是观察到交叉耐药性。它总是与氯霉素耐药相关，并且经常与强力霉素耐药相关。突变体外膜蛋白的电泳改变表明，嗜麦芽窄食单胞菌对喹诺酮类药物的耐药性可能涉及不同的机制。
