Huang Yuan-Pin, Wang Ching-Ho
Department of Veterinary Medicine, National Taiwan University, No 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
Vaccine. 2006 Feb 6;24(6):785-91. doi: 10.1016/j.vaccine.2005.08.081. Epub 2005 Sep 7.
Due to variations in serotypes among different strains of avian infectious bronchitis viruses (IBV), vaccination of chicks with imported vaccines fails to protect them from IBV infections in Taiwan. Therefore, we develop attenuated vaccines from local strains in Taiwan. A Taiwan Group I (TW I) strain was passaged 74 times through specific pathogen-free (SPF) chicken embryonated eggs, and then tested in SPF chickens. The attenuated vaccine was not pathogenic in 1-week-old chicks, had a neutralization index (NI) of greater than 4.4 and efficacy of 90% when inoculated birds were challenged with a field IBV strain. Similar results were obtained for a vaccine made from a Taiwan Group II IBV strain. Additionally, the TW I attenuated vaccine strain had no reversion to virulence after five back passages in chicks. In conclusion, these attenuated vaccines have potential for controlling local Taiwanese IBV infections in chickens.
由于不同株禽传染性支气管炎病毒（IBV）血清型存在差异，在台湾用进口疫苗给雏鸡接种无法保护它们免受IBV感染。因此，我们用台湾本地毒株研制了减毒疫苗。一株台湾I组（TW I）毒株在无特定病原体（SPF）鸡胚中传代74次，然后在SPF鸡中进行测试。该减毒疫苗对1周龄雏鸡无致病性，中和指数（NI）大于4.4，当接种疫苗的鸡受到野外IBV毒株攻击时，疫苗效力达90%。用台湾II组IBV毒株制成的疫苗也得到了类似结果。此外，TW I减毒疫苗株在雏鸡中连续传代五次后未出现毒力返强。总之，这些减毒疫苗有控制台湾本地鸡群IBV感染的潜力。
