Khirwadkar K, Schmitz M, Kabelitz D
Institut für Immunologie, Universität Heidelberg, BRD.
Int Arch Allergy Immunol. 1992;98(1):6-12. doi: 10.1159/000236158.
We have investigated the frequencies of Dermatophagoides pteronyssinus (Dpt)-reactive circulating T cells in individuals allergic to this house dust mite and in asymptomatic controls. In allergic patients the median for frequencies of Dpt-reactive T cells (80/10,000 T cells) was significantly higher (p = 0.0001) than that in the controls (19/10,000 T cells). Frequencies of spontaneously proliferating T cells were also determined. Here too, marked differences (p = 0.0001) were observed between the allergics (median = 32/10,000 T cells) and the non-allergics (median = 7.5/10,000 T cells). The allergen-reactive T cells tended to occur at a higher frequency with increasing serum level of Dpt-specific IgE. Thus the median value for Dpt-reactive T cells in patients showing RAST class greater than or equal to 4 (176.5/10,000 T cells) was higher than that shown by patients with RAST class 3 (62/10,000 T cells). Frequency estimations of allergen-reactive T cells before, during and after the completion of hyposensitization therapy might help to clarify their possible role in the allergic symptoms.
我们研究了对屋尘螨过敏个体及无症状对照者中对粉尘螨（Dpt）产生反应的循环T细胞频率。在过敏患者中，对Dpt产生反应的T细胞频率中位数（80/10,000个T细胞）显著高于对照组（19/10,000个T细胞）（p = 0.0001）。我们还测定了自发增殖T细胞的频率。同样，在过敏者（中位数 = 32/10,000个T细胞）和非过敏者（中位数 = 7.5/10,000个T细胞）之间观察到显著差异（p = 0.0001）。随着Dpt特异性IgE血清水平升高，过敏原反应性T细胞倾向于以更高频率出现。因此，RAST分级大于或等于4级的患者中对Dpt产生反应的T细胞中位数（176.5/10,000个T细胞）高于RAST分级为3级的患者（62/10,000个T细胞）。在减敏治疗前、治疗期间和治疗完成后对过敏原反应性T细胞进行频率估计，可能有助于阐明它们在过敏症状中可能发挥的作用。
