Wang Donggeng, Hang Taijun, Wu Chunyong, Liu Wenying
Department of Pharmaceutical Analysis, China Pharmaceutical University, NanJing 210009, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Dec 27;829(1-2):97-106. doi: 10.1016/j.jchromb.2005.09.040. Epub 2005 Oct 21.
To identify the major metabolites of resveratrol in rat, rat urine samples were pretreated by using solid-phase extraction technique (SPE) with polyamide cartridges. And a LC-MS/MS method with electrospray ionisation (ESI), negative ion mode and collision induced dissociation (CID), was used to elucidate the structures of the major metabolites of resveratrol. According to the results of our experiment, we found that the main metabolites of resveratrol were resveratrol monoglucuronide (M1), dihydroresveratrol monosulfate (M2), resveratrol monosulfate (M3) and dihydroresveratrol (M4).
为了鉴定白藜芦醇在大鼠体内的主要代谢产物，采用聚酰胺柱固相萃取技术（SPE）对大鼠尿液样本进行预处理。并采用电喷雾电离（ESI）、负离子模式和碰撞诱导解离（CID）的液相色谱-串联质谱法（LC-MS/MS）来阐明白藜芦醇主要代谢产物的结构。根据我们的实验结果，我们发现白藜芦醇的主要代谢产物为白藜芦醇单葡萄糖醛酸苷（M1）、二氢白藜芦醇单硫酸盐（M2）、白藜芦醇单硫酸盐（M3）和二氢白藜芦醇（M4）。
