Kang Kyoung Ah, Lee Kyoung Hwa, Chae Sungwook, Zhang Rui, Jung Myung Sun, Lee Youngki, Kim So Young, Kim Hee Sun, Joo Hong Gu, Park Jae Woo, Ham Young Min, Lee Nam Ho, Hyun Jin Won
Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju-si 690-756, Republic of Korea.
FEBS Lett. 2005 Nov 21;579(28):6295-304. doi: 10.1016/j.febslet.2005.10.008. Epub 2005 Oct 19.
We have investigated the cytoprotective effect of eckol, which was isolated from Ecklonia cava, against oxidative stress induced cell damage in Chinese hamster lung fibroblast (V79-4) cells. Eckol was found to scavenge 1,1-diphenyl-2-picrylhydrazyl radical, hydrogen peroxide (H(2)O(2)), hydroxy radical, intracellular reactive oxygen species (ROS), and thus prevented lipid peroxidation. As a result, eckol reduced H(2)O(2) induced cell death in V79-4 cells. In addition, eckol inhibited cell damage induced by serum starvation and radiation by scavenging ROS. Eckol was found to increase the activity of catalase and its protein expression. Further, molecular mechanistic study revealed that eckol increased phosphorylation of extracellular signal-regulated kinase and activity of nuclear factor kappa B. Taken together, the results suggest that eckol protects V79-4 cells against oxidative damage by enhancing the cellular antioxidant activity and modulating cellular signal pathway.
我们研究了从海蕴中分离出的eckol对中国仓鼠肺成纤维细胞（V79-4）中氧化应激诱导的细胞损伤的细胞保护作用。发现eckol能清除1,1-二苯基-2-苦基肼自由基、过氧化氢（H₂O₂）、羟自由基、细胞内活性氧（ROS），从而防止脂质过氧化。结果，eckol减少了H₂O₂诱导的V79-4细胞死亡。此外，eckol通过清除ROS抑制血清饥饿和辐射诱导的细胞损伤。发现eckol能增加过氧化氢酶的活性及其蛋白表达。进一步的分子机制研究表明，eckol增加了细胞外信号调节激酶的磷酸化和核因子κB的活性。综上所述，结果表明eckol通过增强细胞抗氧化活性和调节细胞信号通路来保护V79-4细胞免受氧化损伤。
