de Lichtenberg Ulrik, Wernersson Rasmus, Jensen Thomas Skøt, Nielsen Henrik Bjørn, Fausbøll Anders, Schmidt Peer, Hansen Flemming Bryde, Knudsen Steen, Brunak Søren
Center for Biological Sequence Analysis, BioCentrum, Technical University of Denmark, Building 208, DK-2800 Lyngby, Denmark.
Yeast. 2005 Nov;22(15):1191-201. doi: 10.1002/yea.1302.
We present an approach combining bioinformatics prediction with experimental microarray validation to identify new cell cycle-regulated genes in Saccharomyces cerevisiae. We identify in the order of 100 new cell cycle-regulated genes and show by independent data that these genes in general tend to be more weakly expressed than the genes identified hitherto. Among the genes not previously suggested to be periodically expressed we find genes linked to DNA repair, cell size monitoring and transcriptional control, as well as a number of genes of unknown function. Several of the gene products are believed to be phosphorylated by Cdc28. For many of these new genes, homologues exist in Schizosaccharomyces pombe and Homo sapiens for which the expression also varies with cell cycle progression.
我们提出了一种将生物信息学预测与实验性微阵列验证相结合的方法，以鉴定酿酒酵母中新的细胞周期调控基因。我们鉴定出了约100个新的细胞周期调控基因，并通过独立数据表明，这些基因总体上往往比迄今鉴定出的基因表达更弱。在先前未被认为有周期性表达的基因中，我们发现了与DNA修复、细胞大小监测和转录控制相关的基因，以及一些功能未知的基因。其中一些基因产物被认为可被Cdc28磷酸化。对于许多这些新基因，粟酒裂殖酵母和智人中存在同源物，其表达也随细胞周期进程而变化。
