Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Busan, 619-705, Republic of Korea.
J Microbiol. 2011 Jun;49(3):381-6. doi: 10.1007/s12275-011-0175-5. Epub 2011 Jun 30.
A Gram-staining-negative, motile, non-spore-forming and rod-shaped bacterial strain, 20-23R(T), was isolated from intestine of bensasi goatfish, Upeneus bensasi, and its taxonomic position was investigated by using a polyphasic study. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 20-23R(T) belonged to the genus Shewanella. Strain 20-23R(T) exhibited 16S rRNA gene sequence similarity values of 99.5, 99.2, and 97.5% to Shewanella algae ATCC 51192(T), Shewanella haliotis DW01(T), and Shewanella chilikensis JC5(T), respectively. Strain 20-23R(T) exhibited 93.1-96.0% 16S rRNA gene sequence similarity to the other Shewanella species. It also exhibited 98.3-98.4% gyrB sequence similarity to the type strains of S. algae and S. haliotis. Strain 20-23R(T) contained simultaneously both menaquinones and ubiquinones; the predominant menaquinone was MK-7 and the predominant ubiquinones were Q-8 and Q-7. The fatty acid profiles of strain 20-23R(T), S. algae KCTC 22552(T) and S. haliotis KCTC 12896(T) were similar; major components were iso-C(15:0), C(16:0), C(16:1) ω7c and/or iso-C(15:0) 2-OH and C(17:1) ω8c. The DNA G+C content of strain 20-23R(T) was 53.9 mol%. Differential phenotypic properties and genetic distinctiveness of strain 20-23R(T), together with the phylogenetic distinctiveness, revealed that this strain is distinguishable from recognized Shewanella species. On the basis of the data presented, strain 20-23R(T) represents a novel species of the genus Shewanella, for which the name Shewanella upenei sp. nov. is proposed. The type strain is 20-23R(T) (=KCTC 22806(T) =CCUG 58400(T)).
一株革兰氏阴性、运动、非孢子形成和杆状的细菌菌株 20-23R(T) 从 bensasi 石斑鱼（Upeneus bensasi）的肠道中分离出来，通过多相研究来研究其分类地位。基于 16S rRNA 基因序列的系统发育分析表明，菌株 20-23R(T) 属于希瓦氏菌属。菌株 20-23R(T) 与 Shewanella algae ATCC 51192(T)、Shewanella haliotis DW01(T) 和 Shewanella chilikensis JC5(T) 的 16S rRNA 基因序列相似性分别为 99.5%、99.2%和 97.5%。菌株 20-23R(T) 与其他希瓦氏菌属的 16S rRNA 基因序列相似性为 93.1-96.0%。它还与 S. algae 和 S. haliotis 的模式株具有 98.3-98.4%的 gyrB 序列相似性。菌株 20-23R(T) 同时含有甲萘醌和泛醌；主要的甲萘醌是 MK-7，主要的泛醌是 Q-8 和 Q-7。菌株 20-23R(T)、Shewanella algae KCTC 22552(T) 和 Shewanella haliotis KCTC 12896(T) 的脂肪酸图谱相似；主要成分是 iso-C(15:0)、C(16:0)、C(16:1) ω7c 和/或 iso-C(15:0) 2-OH 和 C(17:1) ω8c。菌株 20-23R(T) 的 DNA G+C 含量为 53.9 mol%。菌株 20-23R(T) 的表型特征差异和遗传独特性以及系统发育上的独特性表明，该菌株与公认的希瓦氏菌属物种不同。基于所提供的数据，菌株 20-23R(T) 代表希瓦氏菌属的一个新种，建议将其命名为 Shewanella upenei sp. nov.。模式菌株为 20-23R(T) (=KCTC 22806(T) =CCUG 58400(T))。
