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采用可溶性细胞提取物凝胶电泳对乳链球菌进行分组。

Grouping of lactic streptococci by gel electrophoresis of soluble cell extracts.

机构信息

New Zealand Dairy Research Institute, Palmerston North, New Zealand.

出版信息

Appl Environ Microbiol. 1979 Mar;37(3):391-8. doi: 10.1128/aem.37.3.391-398.1979.

DOI:10.1128/aem.37.3.391-398.1979
PMID:16345352
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC243227/
Abstract

Soluble cell proteins obtained from 35 strains of lactic streptococci were examined by gel electrophoresis. A mathematical analysis of the densitometer scans of the gels enabled strains to be grouped according to their overall similarity. Strains which were known to be variants of the same parent strain fell into the same group, supporting the validity of the method. It is suggested that strains which are alike according to their gel electrophoretic patterns when grown under standard conditions have an overall phenotypic similarity and that this indicates a similarity in genotype. The relevance of this to selection of strains of lactic streptococci for cheesemaking is discussed.

摘要

从 35 株乳链球菌中提取的可溶性细胞蛋白通过凝胶电泳进行了检查。通过对凝胶密度计扫描的数学分析,可以根据菌株的整体相似性对其进行分组。已知是同一亲本菌株的变体的菌株归入同一组,支持了该方法的有效性。建议在标准条件下生长时,根据其凝胶电泳模式相似的菌株具有整体表型相似性,这表明基因型相似。讨论了这种方法与选择用于制作奶酪的乳链球菌菌株的相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c2/243227/2ceb993e6e97/aem00207-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c2/243227/2ceb993e6e97/aem00207-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c2/243227/2ceb993e6e97/aem00207-0049-a.jpg

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本文引用的文献

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Differentiation of Campylobacter species by protein banding patterns in polyacrylamide slab gels.通过聚丙烯酰胺平板凝胶中的蛋白质条带模式对弯曲杆菌属菌种进行鉴别。
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Differentiation of Lactococcus lactis and Lactococcus garvieae from humans by comparison of whole-cell protein patterns.通过比较全细胞蛋白图谱区分来自人类的乳酸乳球菌和格氏乳球菌。
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