Elliott J A, Collins M D, Pigott N E, Facklam R R
Division of Bacterial and Mycotic Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1991 Dec;29(12):2731-4. doi: 10.1128/jcm.29.12.2731-2734.1991.
We tested 12 reference and 24 clinical strains of lactococci for physiologic characteristics using a conventional test system, the Gen-Probe Enterococcus 2 chemiluminescence assay (Gen-Probe Inc., San Diego, Calif.), the Rapid Strep identification system (Analytab Products, Plainview, N.Y.), and whole-cell protein analysis. The Gen-Probe Enterococcus 2 chemiluminescence assay for Enterococcus identification was negative with all strains. Neither the conventional test nor the Rapid Strep identification system could differentiate between the two Lactococcus spp. most commonly isolated from humans. A simple procedure, based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was developed for comparing the whole-cell protein patterns of Lactococcus spp. L. lactis and L. garvieae were differentiated by unique protein patterns.
我们使用传统检测系统、Gen-Probe肠球菌2化学发光检测法（Gen-Probe公司，加利福尼亚州圣地亚哥）、快速链球菌鉴定系统（Analytab Products公司，纽约州普莱恩维尤）以及全细胞蛋白分析，对12株乳酸球菌参考菌株和24株临床菌株的生理特性进行了检测。Gen-Probe肠球菌2化学发光检测法对所有菌株进行肠球菌鉴定均呈阴性。传统检测方法和快速链球菌鉴定系统均无法区分最常从人类分离出的两种乳酸球菌。我们开发了一种基于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳的简单方法，用于比较乳酸球菌的全细胞蛋白图谱。乳酸乳球菌和格氏乳球菌可通过独特的蛋白图谱进行区分。
