Panganiban A T, Patt T E, Hart W, Hanson R S
Appl Environ Microbiol. 1979 Feb;37(2):303-9. doi: 10.1128/aem.37.2.303-309.1979.
Methane was oxidized to carbon dioxide in the absence of oxygen by water samples from Lake Mendota, Madison, Wis. The anaerobic oxidation of methane did not result in the assimilation of carbon from methane into material precipitable by cold 10% trichloracetic acid. Only samples taken at the suface of the sediment of Lake Mendota were capable of catalyzine the anaerobic oxidation of methane. The rate of methane oxidation in the presence of oxygen was highest in samples taken from near the thermocline. Of the radioactive methane oxidized, 30 to 60% was assimilated into material precipitable by cold 10% trichloroacetic acid during aerobic incubation of the samples. These data support the conclusion that two distinct groups of methane-oxidizing organisms occur in stratifield lakes. Enrichments with acetate and methane as the sole sources of carbon and energy and sulfate as the electron acceptor resulted in the growth of bacteria that oxidize methane. Sulfate, acetate, and methane were all required for growth of enrichments. Acetate was not oxidized to carbon dioxide but was assimilated by cells. Methane was not assimilated but was oxidized to carbon dioxide in the absence of air.
在无氧条件下，来自威斯康星州麦迪逊市门多塔湖的水样可将甲烷氧化为二氧化碳。甲烷的厌氧氧化并未导致甲烷中的碳被同化为可被冷的10%三氯乙酸沉淀的物质。只有从门多塔湖沉积物表面采集的样品能够催化甲烷的厌氧氧化。在有氧条件下，甲烷氧化速率在从温跃层附近采集的样品中最高。在样品的需氧培养过程中，被氧化的放射性甲烷中有30%至60%被同化为可被冷的10%三氯乙酸沉淀的物质。这些数据支持了这样一个结论，即在分层湖泊中存在两组不同的甲烷氧化生物。以乙酸盐和甲烷作为唯一的碳源和能源，以硫酸盐作为电子受体进行富集培养，导致了氧化甲烷的细菌生长。硫酸盐、乙酸盐和甲烷都是富集培养生长所必需的。乙酸盐未被氧化为二氧化碳，而是被细胞同化。甲烷未被同化，但在无空气的情况下被氧化为二氧化碳。
