丙酮丁醇发酵生产菌在合成培养基中的生产。
Acetone and Butanol Production by Clostridium acetobutylicum in a Synthetic Medium.
机构信息
Laboratoire des Sciences du Génie Chimique, CNRS-ENSIC, 54042 Nancy, Cedex, France, and Université de Nancy I, Laboratoire de Chimie Biologique I, 54037 Nancy, Cedex, France.
出版信息
Appl Environ Microbiol. 1982 Dec;44(6):1318-24. doi: 10.1128/aem.44.6.1318-1324.1982.
Abstract
The effect of the component concentrations of a synthetic medium on acetone and butanol fermentation by Clostridium acetobutylicum ATCC 824 was investigated. Cell growth was dependent on the presence of Mg, Fe, and K in the medium. Mg and Mn had deleterious effects when in excess. Ammonium acetate in excess caused acid fermentation. The metabolism was composed of two phases: an acid phase and a solvent one. Low concentrations of glucose allowed the first phase only. The theoretical ratio of the conversion of glucose to solvents, which was 28 to 33%, was obtained with the following medium: MgSO(4), 50 to 200 mg/liter; MnSO(4), 0 to 20 mg/liter; KCl, 0.015 to 8 g/liter (an equivalent concentration of K was supplied in the form of KH(2)PO(4) and K(2)HPO(4)); FeSO(4), 1 to 50 mg/liter; ammonium acetate, 1.1 to 2.2 g/liter; para-aminobenzoic acid, 1 mg/liter; biotin, 0.01 mg/liter; glucose, 20 to 60 g/liter.
摘要
研究了合成培养基中各成分浓度对丙酮丁醇梭菌 ATCC824 发酵丙酮和丁醇的影响。细胞生长依赖于培养基中镁、铁和钾的存在。过量的镁和锰会产生有害影响。过量的乙酸铵会导致酸发酵。该代谢过程由两个阶段组成：酸相和溶剂相。低浓度的葡萄糖仅允许进行第一阶段。用以下培养基可获得葡萄糖到溶剂的理论转化率为 28%至 33%：硫酸镁，50 至 200 毫克/升；硫酸锰，0 至 20 毫克/升；氯化钾，0.015 至 8 克/升（以 KH2PO4 和 K2HPO4 的形式提供相当于 K 的浓度）；硫酸亚铁，1 至 50 毫克/升；乙酸铵，1.1 至 2.2 克/升；对氨基苯甲酸，1 毫克/升；生物素，0.01 毫克/升；葡萄糖，20 至 60 克/升。
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