Department of Applied Microbiology and Food Science, College of Agriculture, and the Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 0W0.
Appl Environ Microbiol. 1983 Mar;45(3):862-7. doi: 10.1128/aem.45.3.862-867.1983.
A Fusarium metabolite, T-2 toxin, inhibits the growth of Saccharomyces carlsbergensis and Saccharomyces cerevisiae. The growth inhibitory concentrations of T-2 toxin were 40 and 100 mug/ml, respectively, for exponentially growing cultures of the two yeasts. S. carlsbergensis was more sensitive to the toxin and exhibited a biphasic dose-response curve. Addition of the toxin at 10 mug/ml of S. carlsbergensis culture resulted in a retardation of growth as measured turbidimetrically, after only 30 to 40 min. This action was reversible upon washing the cells free of the toxin. The sensitivity of the yeasts to the toxin was dependent upon the types and concentrations of carbohydrates used in the growth media. The sensitivity of the cells to the toxin decreased in glucose-repressed cultures. These results suggest that T-2 toxin interferes with mitochondrial functions of these yeasts.
镰刀菌代谢产物 T-2 毒素抑制酿酒酵母和酿酒酵母的生长。T-2 毒素对两种酵母指数生长期培养物的生长抑制浓度分别为 40 和 100μg/ml。酿酒酵母对毒素更敏感，并表现出双相剂量反应曲线。在添加 10μg/ml 的 T-2 毒素到酿酒酵母培养物中后，仅 30 至 40 分钟后，用比浊法测量的生长就会延迟。当用毒素清洗细胞时，这种作用是可逆的。酵母对毒素的敏感性取决于生长培养基中使用的碳水化合物的类型和浓度。在葡萄糖抑制的培养物中，细胞对毒素的敏感性降低。这些结果表明 T-2 毒素干扰了这些酵母的线粒体功能。
