Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, New York 12201-0509.
Appl Environ Microbiol. 1988 Nov;54(11):2655-9. doi: 10.1128/aem.54.11.2655-2659.1988.
The hydrolysis and fermentation of insoluble cellulose (Avicel) by continuous cultures of Ruminococcus albus 7 was studied. An anaerobic continuous culture apparatus was designed which permitted gas collection, continuous feeding, and wasting at different retention times. The operation of the apparatus was controlled by a personal computer. Cellulose destruction ranged from ca. 30 to 70% for hydraulic retention times of 0.5 to 2.0 days. Carbon recovery in products was 92 to 97%, and the oxidation-reduction ratios ranged from 0.91 to 1.15. The total product yield (biomass not included) per gram of cellulose (expressed as glucose) was 0.83 g g, and the ethanol yield was 0.41 g g. The product yield was constant, indicating that product formation was growth linked.
我们研究了不溶性纤维素（Avicel）在白色瘤胃球菌 7 的连续培养物中的水解和发酵。我们设计了一种厌氧连续培养装置，该装置允许在不同的保留时间进行气体收集、连续进料和浪费。该设备的运行由个人计算机控制。水力停留时间为 0.5 至 2.0 天时，纤维素的破坏率约为 30%至 70%。产物中的碳回收率为 92%至 97%，氧化还原比范围为 0.91 至 1.15。每克纤维素（以葡萄糖表示）的总产物（不包括生物质）产率为 0.83 gg，乙醇产率为 0.41 gg。产物产率是恒定的，表明产物形成与生长有关。
