Swedish Environmental Research Institute, Box 21060, S-100 31 Stockholm, Sweden.
Appl Environ Microbiol. 1991 Jan;57(1):77-84. doi: 10.1128/aem.57.1.77-84.1991.
Metabolically stable anaerobic cultures obtained by enrichment with 5-bromovanillin, 5-chlorovanillin, catechin, and phloroglucinol were used to study dechlorination of chlorocatechols. A high degree of specificity in dechlorination was observed, and some chlorocatechols were appreciably more resistant to dechlorination than others: only 3,5-dichlorocatechol, 4,5-dichlorocatechol, 3,4,5-trichlorocatechol, and tetrachlorocatechol were dechlorinated, and not all of them were dechlorinated by the same consortium. 3,5-Dichlorocatechol produced 3-chlorocatechol, 4,5-dichlorocatechol produced 4-chlorocatechol, and 3,4,5-trichlorocatechol produced either 3,5-dichlorocatechol or 3,4-dichlorocatechol; tetrachlorocatechol produced only 3,4,6-trichlorocatechol. Incubation of uncontaminated sediments without additional carbon sources brought about dechlorination of 3,4,5-trichlorocatechol to 3,5-dichlorocatechol. O-demethylation of chloroguaiacols was generally accomplished by enrichment cultures, except that catechin enrichment was unable to O-demethylate tetrachloroguaiacol. None of the enrichments dechlorinated any of the polychlorinated phenols examined. The results suggested that dechlorination was not dependent on enrichment with or growth at the expense of chlorinated compounds and that it would be premature to formulate general rules for the structural dependence of the dechlorination reaction.
用 5-溴藜芦醛、5-氯藜芦醛、儿茶素和间苯三酚进行富集获得代谢稳定的厌氧培养物，用于研究氯代邻苯二酚的脱氯。观察到脱氯具有高度的特异性，一些氯代邻苯二酚比其他氯代邻苯二酚更能抵抗脱氯：只有 3,5-二氯邻苯二酚、4,5-二氯邻苯二酚、3,4,5-三氯邻苯二酚和四氯邻苯二酚被脱氯，并且并非所有这些氯代邻苯二酚都被同一共生体脱氯。3,5-二氯邻苯二酚产生 3-氯邻苯二酚，4,5-二氯邻苯二酚产生 4-氯邻苯二酚，3,4,5-三氯邻苯二酚产生 3,5-二氯邻苯二酚或 3,4-二氯邻苯二酚；四氯邻苯二酚仅产生 3,4,6-三氯邻苯二酚。在没有添加碳源的未污染沉积物中孵育导致 3,4,5-三氯邻苯二酚脱氯生成 3,5-二氯邻苯二酚。除儿茶素富集不能对四氯邻苯三酚进行 O-脱甲基化外，氯代愈创木酚的 O-脱甲基化通常由富集培养物完成。没有任何一种富集物能脱氯任何一种所检查的多氯苯酚。结果表明，脱氯不依赖于用氯化化合物富集或生长，并且制定脱氯反应结构依赖性的一般规则还为时过早。
