Fathepure B Z, Tiedje J M, Boyd S A
Department of Crop and Soil Sciences, Michigan State University, East Lansing 48824.
Appl Environ Microbiol. 1988 Feb;54(2):327-30. doi: 10.1128/aem.54.2.327-330.1988.
Hexachlorobenzene was dechlorinated to tri- and dichlorobenzenes in anaerobic sewage sludge. The complete biotransformation of 190 microM hexachlorobenzene (approximately 50 ppm) occurred within 3 weeks. The calculated rate of hexachlorobenzene dechlorination was 13.6 mumol liter-1 day-1. Hexachlorobenzene was dechlorinated via two routes, both involving the sequential removal of chlorine from the aromatic ring. The major route was hexachlorobenzene----pentachlorobenzene----1,2,3,5-tetrachlorobenzene--- -1,3,5- trichlorobenzene. Greater than 90% of the added hexachlorobenzene was recovered as 1,3,5-trichlorobenzene, and there was no evidence for further dechlorination of 1,3,5-trichlorobenzene. The minor route was hexachlorobenzene----pentachlorobenzene----1,2,4,5-tetrachlorobenzene--- -1,2,4- trichlorobenzene----dichlorobenzenes. These results extend reductive dechlorination to poorly water soluble aromatic hydrocarbons which could potentially include other important environmental pollutants like polychlorinated biphenyls.
六氯苯在厌氧污水污泥中被脱氯生成三氯苯和二氯苯。190微摩尔（约50 ppm）的六氯苯在3周内完全生物转化。计算得出的六氯苯脱氯速率为13.6微摩尔·升⁻¹·天⁻¹。六氯苯通过两条途径脱氯，两条途径均涉及从芳环上依次去除氯原子。主要途径是六氯苯→五氯苯→1,2,3,5 - 四氯苯→1,3,5 - 三氯苯。添加的六氯苯中超过90%以1,3,5 - 三氯苯的形式回收，没有证据表明1,3,5 - 三氯苯会进一步脱氯。次要途径是六氯苯→五氯苯→1,2,4,5 - 四氯苯→1,2,4 - 三氯苯→二氯苯。这些结果将还原脱氯作用扩展到了水溶性差的芳烃，其中可能包括多氯联苯等其他重要的环境污染物。
