Laboratoire de Biologie Cellulaire Fongique, UMR Centre National de la Recherche Scientifique 106, Université Lyon I (Bât. 405), 43, bd. du 11 Novembre 1918, 69622 Villeurbanne Cedex, and Rhône Poulenc Agrochimie, Service de Biologie Moléculaire et Cellulaire Végétales, 69263 Lyon Cedex 09, France.
Appl Environ Microbiol. 1991 May;57(5):1478-84. doi: 10.1128/aem.57.5.1478-1484.1991.
The range of polysaccharide-degrading enzymes and glycosidases formed by the phytopathogenic fungus Sclerotinia sclerotiorum was monitored following growth on 16 carbohydrate substrates. Endo- and exoenzymes capable of degrading cellulosic, hemicellulosic, and pectinolytic polysaccharides were secreted. Pectinolytic activities were produced constitutively on all of the substrates tested. Cellulolytic enzymes were not induced in simple sugar (i.e., glucose or xylose) media. Polysaccharide growth substrates and cellulase inducers increased all of the enzyme activities tested. Gel filtration analysis revealed the appearance of new molecular forms of pectinase, beta-xylosidase, and cellobiosidase during induction on pectin and carboxymethyl cellulose media.
在以 16 种碳水化合物为底物进行生长后，监测了植物病原真菌核盘菌形成的多糖降解酶和糖苷酶的范围。能够降解纤维素、半纤维素和果胶多糖的内切酶和外切酶被分泌出来。在所有测试的底物上，果胶酶活性都是组成型产生的。在简单糖（即葡萄糖或木糖）培养基中，不诱导纤维素酶。多糖生长底物和纤维素酶诱导剂增加了所有测试的酶活性。凝胶过滤分析显示，在果胶和羧甲基纤维素培养基上诱导时，出现了新的果胶酶、β-木糖苷酶和纤维二糖酶的分子形式。
