Department of General Microbiology, University of Copenhagen, Sølvgade 83 H, DK-1307 Copenhagen K, Denmark.
Appl Environ Microbiol. 1992 Aug;58(8):2660-2. doi: 10.1128/aem.58.8.2660-2662.1992.
The incorporation of [H]thymidine by axenic cultures of anaerobic bacteria was investigated as a means to measure growth. The three fermentative strains and one of the methanogenic strains tested incorporated [H]thymidine, whereas the sulfate-reducing bacterium and two of the methanogenic bacteria were unable to incorporate [H]thymidine during growth. It is concluded that the [H]thymidine incorporation method underestimates bacterial growth in anaerobic environments.
本研究采用[H]胸苷掺入法来检测厌氧菌的生长情况。在所测试的 3 株发酵菌和 1 株产甲烷菌中，均可检测到[H]胸苷的掺入，而硫酸盐还原菌和 2 株产甲烷菌在生长过程中则无法掺入[H]胸苷。由此可见，[H]胸苷掺入法可能低估了厌氧环境中的细菌生长。
