Department of General Microbiology, University of Copenhagen, Sølvgade 83 H, DK-1307 Copenhagen, Denmark.
Appl Environ Microbiol. 1991 Sep;57(9):2453-8. doi: 10.1128/aem.57.9.2453-2458.1991.
We studied the degradation of pentachlorophenol (PCP) under methanogenic and sulfate-reducing conditions with an anaerobic mixed culture derived from sewage sludge. The consortium degraded PCP via 2,3,4,5-tetrachlorophenol, 3,4,5-trichlorophenol, and 3,5-dichlorophenol and eventually accumulated 3-chlorophenol. Dechlorination of PCP and metabolites was inhibited in the presence of sulfate, thiosulfate, and sulfite. A decrease in the rate of PCP transformation was noted when the endogenous dissolved H(2) was depleted below 0.11 muM in sulfate-reducing cultures. The effect on dechlorination observed with sulfate could be relieved by addition of molybdate, a competitive inhibitor of sulfate reduction. Addition of H(2) reduced the inhibition observed with sulfuroxy anions. The inhibitory effect of sulfuroxy anions may be due to a competition for H(2) between sulfate reduction and dechlorination. When cultured under methanogenic conditions, the consortium degraded several chlorinated and brominated phenols.
我们研究了来自污水污泥的厌氧混合培养物在产甲烷和硫酸盐还原条件下对五氯苯酚（PCP）的降解。该联合体通过 2,3,4,5-四氯苯酚、3,4,5-三氯苯酚和 3,5-二氯苯酚降解 PCP，并最终积累 3-氯苯酚。在硫酸盐、硫代硫酸盐和亚硫酸盐存在下，PCP 和代谢物的脱氯作用受到抑制。在硫酸盐还原培养物中，当内源性溶解 H（2）消耗至低于 0.11 μM 时，PCP 转化的速率降低。硫酸盐对脱氯的影响可以通过添加钼酸盐（硫酸盐还原的竞争性抑制剂）来缓解。添加 H（2）减少了与硫氧阴离子观察到的抑制作用。硫氧阴离子的抑制作用可能是由于硫酸盐还原和脱氯之间对 H（2）的竞争。在产甲烷条件下培养时，联合体降解了几种氯化和溴化酚。
