Prema B R, Bhattacharyya P K
National Chemical Laboratory, Poona, India.
Appl Microbiol. 1962 Nov;10(6):524-8. doi: 10.1128/am.10.6.524-528.1962.
Several strains of fungi were tested for their ability to metabolize alpha-pinene in shake cultures. A strain of Aspergillus niger showing marked efficiency in this respect was selected for further studies. The optimal conditions for fermentation were established with respect to substrate concentration, time, and temperature. From the fermentation products three major metabolites of alpha-pinene were isolated: a ketone, C(10)H(14)O, identified as d-verbenone; an alcohol, C(10)H(16)O, identified as d-cis-verbenol; and a crystalline diol, C(10)H(18)O(2), characterized as d-trans-sobrerol.
对几种真菌菌株进行了测试,以考察它们在摇瓶培养中代谢α-蒎烯的能力。挑选出了在这方面表现出显著效率的黑曲霉菌株用于进一步研究。针对底物浓度、时间和温度确定了发酵的最佳条件。从发酵产物中分离出了α-蒎烯的三种主要代谢物:一种酮,C(10)H(14)O,鉴定为d-马鞭草烯酮;一种醇,C(10)H(16)O,鉴定为d-顺式马鞭草烯醇;以及一种结晶二醇,C(10)H(18)O(2),特征为d-反式异龙脑。