荧光抗体法与富集血清学检测沙门氏菌的比较
Comparison of fluorescent-antibody methods and enrichment serology for the detection of Salmonella.
作者信息
Mohr H K, Trenk H L, Yeterian M
出版信息
Appl Microbiol. 1974 Feb;27(2):324-8. doi: 10.1128/am.27.2.324-328.1974.
Abstract
Four rapid methods for detection of Salmonella, (i) the conventional fluorescent-antibody (FA) technique, (ii) a rapid direct FA technique, (iii) microcolony FA, and (iv) enrichment serology (ES), were compared with conventional cultural procedures. A total of 347 subsamples representing 16 different food prototypes, alleged to be naturally contaminated with Salmonella, were analyzed. From these samples, 52 were found to contain Salmonella by cultural methods. Conventional FA identified all 52 culturally positive samples, ES identified 51, microcolony FA identified 48, and the rapid FA method identified 34. The number of false-positive samples for each procedure was: ES-selenite, 7; tetrathionate, 8; rapid FA, 26; microcolony FA, 33; conventional FA-selenite, 27; tetrathionate, 26. Tetrathionate enrichment was found to be superior to selenite for Salmonella recovery from most foods, but the concurrent use of both media allowed maximum recovery.
摘要
比较了四种检测沙门氏菌的快速方法，（i）传统荧光抗体（FA）技术，（ii）快速直接FA技术，（iii）微菌落FA，以及（iv）增菌血清学（ES），并与传统培养方法进行了比较。总共分析了代表16种不同食品原型的347个亚样本，据称这些样本自然受到沙门氏菌污染。通过培养方法从这些样本中发现52个含有沙门氏菌。传统FA鉴定出所有52个培养阳性样本，ES鉴定出51个，微菌落FA鉴定出48个，快速FA方法鉴定出34个。每种方法的假阳性样本数量为：ES-亚硒酸盐7个；四硫磺酸盐8个；快速FA 26个；微菌落FA 33个；传统FA-亚硒酸盐27个；四硫磺酸盐26个。发现从大多数食品中回收沙门氏菌时，四硫磺酸盐增菌优于亚硒酸盐，但同时使用两种培养基可实现最大回收率。
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