Karmysheva V Ia, Rovenskiĭ Iu A, Mironova O I, Koniushko O I, Khapchaev Iu Kh
Tsitologiia. 1992;34(1):84-8.
Five nontumorogenic cell lines suitable for vaccine production were studied by SEM. It was shown that diploid cell line 41 originated from sheep embryo kidney and also two heteroploid cell lines, line 4921 originated from embryo skin and muscles of the African green young monkey and line 4647 from kidney of the adult monkey, maintained normal cell morphology and normal growth pattern in early and in later passages in cultures. Some alterations in epithelial dense monolayer formation were revealed in the heteroploid cell lines: in line 455 originated from spleen of the adult African green monkey, and in line 4184 originated from line 41. The revealed alterations can be considered as the early morphological signs of the transformation of epithelial cells in culture. These cell lines also retained the stability of their morphological characteristics at the earlier and later passages. All the studied cell lines were free of contaminating agents.
通过扫描电子显微镜对五种适合疫苗生产的非致瘤细胞系进行了研究。结果表明，源自绵羊胚胎肾的二倍体细胞系41，以及源自非洲绿猴幼体胚胎皮肤和肌肉的异倍体细胞系4921和源自成年猴肾脏的细胞系4647，在培养的早期和后期传代中均保持正常的细胞形态和正常的生长模式。在异倍体细胞系中发现了上皮致密单层形成的一些改变：源自成年非洲绿猴脾脏的细胞系455，以及源自细胞系41的细胞系4184。所揭示的改变可被视为培养上皮细胞转化的早期形态学迹象。这些细胞系在早期和后期传代时也保持了其形态特征的稳定性。所有研究的细胞系均无污染物。
