Nierenberg D W, Nann S L
Department of Medicine, Dartmouth Medical School, Lebanon, NH 03756.
Am J Clin Nutr. 1992 Aug;56(2):417-26. doi: 10.1093/ajcn/56.2.417.
To explore the relation between plasma and tissue concentrations of seven fat-soluble micronutrients (retinol, alpha-tocopherol, lutein, cryptoxanthin, lycopene, alpha-carotene, and beta-carotene), we developed methods for clarifying plasma and tissue samples and quantitating these seven analytes simultaneously by using HPLC with ultraviolet detection. Clarification of tissue samples was performed by using enzymatic digestion followed by mechanical homogenization; saponification was avoided and tocopherol nicotinate was used as an internal standard. Precision and sensitivity for small samples of plasma and solid tissues were determined. Accuracy for plasma samples was assessed by comparing results obtained by using this method with those obtained by using older, standardized methods. Results from application of this method to patient samples of plasma and various tissues are presented. This method will be of interest to investigators seeking to quantitate these seven micronutrients in solid tissue samples, but desiring to avoid the usual saponification step required in most other reported techniques.
为了探究七种脂溶性微量营养素（视黄醇、α-生育酚、叶黄素、隐黄质、番茄红素、α-胡萝卜素和β-胡萝卜素）的血浆浓度与组织浓度之间的关系，我们开发了一些方法，用于澄清血浆和组织样本，并通过使用带紫外检测的高效液相色谱法同时对这七种分析物进行定量。组织样本的澄清通过酶消化后进行机械匀浆来完成；避免了皂化反应，并使用生育酚烟酸酯作为内标。测定了血浆和实体组织小样本的精密度和灵敏度。通过将该方法所得结果与使用旧的标准化方法所得结果进行比较，评估了血浆样本的准确性。展示了该方法应用于患者血浆和各种组织样本的结果。对于那些试图在实体组织样本中定量这七种微量营养素，但又希望避免大多数其他报道技术中通常所需的皂化步骤的研究人员来说，该方法将会很有意义。
