Wilkinson J M
Biochem J. 1969 Apr;112(2):173-85. doi: 10.1042/bj1120173.
The sequences of the N-terminal peptides prepared by Pronase digestion of the heavy chain of rabbit immunoglobulin G of allotype Aa1, Aa2 and Aa3 were determined and were shown to be related to the allotype. An N-terminal fragment of about 34 residues was also prepared from the allotype heavy chains, by cleavage with cyanogen bromide; the yield varied with the allotype. The sequences of the cyanogen bromide fragments from the Aa1 and Aa3 heavy chains contain allotype-related variations similar to those found in the N-terminal Pronase peptides, and these sequences are thought to be representative of the whole heavy-chain populations. There is about 60% homology between the two sequences, and superimposed on the differences between them there are a number of positions within each sequence at which at least two amino acids are present.
通过胰凝乳蛋白酶消化同种异型Aa1、Aa2和Aa3的兔免疫球蛋白G重链所制备的N端肽段的序列已被确定，并显示与同种异型有关。还通过溴化氰裂解从同种异型重链制备了一个约34个残基的N端片段；产率因同种异型而异。来自Aa1和Aa3重链的溴化氰片段的序列包含与在N端胰凝乳蛋白酶肽段中发现的类似的与同种异型相关的变异，并且这些序列被认为代表了整个重链群体。这两个序列之间存在约60%的同源性，并且在它们之间的差异之上，每个序列内有许多位置存在至少两种氨基酸。
