Liu Yan, Xu Yufang, Qian Xuhong, Liu Jianwen, Shen Liyun, Li Junhui, Zhang Yuanxing
School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
Bioorg Med Chem. 2006 May 1;14(9):2935-41. doi: 10.1016/j.bmc.2005.12.012. Epub 2006 Jan 5.
Novel naphthalimides with two heterocyclic side chains of 2-nitroimidazole for bioreductive binding were designed, synthesized, and used as fluorescent markers for hypoxic cells. Their evaluation for imaging tumor hypoxia was carried out in V79 cells, CHO cells, and 95D cells in vitro by using fluorescence scan ascent. A2 and A4 showed a very large differential fluorescence between hypoxic and oxic cells (V79 cells) in vitro and are promising candidate markers for hypoxic cells.
设计、合成了带有两个用于生物还原结合的2-硝基咪唑杂环侧链的新型萘二甲酰亚胺，并将其用作缺氧细胞的荧光标记物。通过荧光扫描上升法在体外对V79细胞、CHO细胞和95D细胞中它们对肿瘤缺氧成像的性能进行了评估。A2和A4在体外缺氧和有氧细胞（V79细胞）之间显示出非常大的荧光差异，是很有前景的缺氧细胞候选标记物。
