Boyce John D, Adler Ben
Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Australian Bacterial Pathogenesis Program, Monash University, Victoria 3800, Australia.
Curr Opin Microbiol. 2006 Feb;9(1):117-22. doi: 10.1016/j.mib.2005.12.005. Epub 2006 Jan 6.
Pasteurella multocida is a Gram-negative bacterial pathogen, which causes diseases of economic importance in a wide range of animal species. The response of P. multocida to the host environment has been analysed at the transcription level, using DNA microarrays, and at the protein-expression level, using proteomics techniques. Furthermore, a growing number of P. multocida-directed mutants have been assessed for their ability to cause disease. Although technical impediments mean that it is currently difficult to analyse bacterial responses at the earliest stages of infection, it is clear that during later stages of infection the bacteria encounter host niches that require them to modify the expression of genes involved in central energy metabolism and in the uptake of various nutrients such as iron and amino acids. Furthermore, in vitro experiments have defined the varying bacterial responses to low iron and to different iron sources, including haemoglobin and transferrin. To date, most P. multocida genes shown to be upregulated during infection are involved in nutrient acquisition and metabolic processes, indicating that true virulence genes might be constitutively expressed, upregulated only during initial stages of infection or upregulated at levels below current detection limits.
多杀性巴氏杆菌是一种革兰氏阴性细菌病原体，可在多种动物物种中引发具有经济重要性的疾病。已使用DNA微阵列在转录水平以及使用蛋白质组学技术在蛋白质表达水平分析了多杀性巴氏杆菌对宿主环境的反应。此外，越来越多针对多杀性巴氏杆菌的突变体已被评估其致病能力。尽管技术障碍意味着目前在感染的最早阶段难以分析细菌反应，但很明显，在感染后期，细菌会遇到宿主生态位，这要求它们改变参与中心能量代谢以及摄取各种营养物质（如铁和氨基酸）的基因的表达。此外，体外实验已经确定了细菌对低铁和不同铁源（包括血红蛋白和转铁蛋白）的不同反应。迄今为止，大多数在感染期间显示上调的多杀性巴氏杆菌基因都参与营养获取和代谢过程，这表明真正的毒力基因可能是组成性表达的，仅在感染初始阶段上调或上调至低于当前检测限的水平。
