Priya G Bhuvana, Nagaleekar Viswas Konasagara, Milton A Arun Prince, Saminathan M, Kumar Amod, Sahoo Amit Ranjan, Wani Sajad Ahmad, Kumar Amit, Gupta S K, Sahoo Aditya P, Tiwari A K, Agarwal R K, Gandham Ravi Kumar
Division of Bacteriology & Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
PLoS One. 2017 Jul 13;12(7):e0179420. doi: 10.1371/journal.pone.0179420. eCollection 2017.
Pasteurella multocida causes acute septicemic and respiratory diseases, including haemorrhagic septicaemia, in cattle and buffalo with case fatality of 100%. In the present study, mice were infected with P. multocida (1.6 × 103 cfu, intraperitoneal) to evaluate host gene expression profile at early and late stages of infection using high throughput microarray transcriptome analyses. Several differentially expressed genes (DEGs) at both the time points were identified in P.multocida infected spleen, liver and lungs. Functional annotation of these DEGs showed enrichment of key pathways such as TLR, NF-κB, MAPK, TNF, JAK-STAT and NOD like receptor signaling pathways. Several DEGs overlapped across different KEGG pathways indicating a crosstalk between them. The predicted protein-protein interaction among these DEGs suggested, that the recognition of P. multocida LPS or outer membrane components by TLR4 and CD14, results in intracellular signaling via MyD88, IRAKs and/or TRAF6 leading to activation of NFκB and MAPK pathways and associated cytokines.
多杀性巴氏杆菌可引发牛和水牛的急性败血性及呼吸道疾病，包括出血性败血症，病死率达100%。在本研究中，用多杀性巴氏杆菌（1.6×10³ 菌落形成单位，腹腔注射）感染小鼠，通过高通量微阵列转录组分析评估感染早期和晚期宿主基因表达谱。在多杀性巴氏杆菌感染的脾脏、肝脏和肺中，鉴定出了两个时间点的几个差异表达基因（DEG）。这些DEG的功能注释显示关键通路如TLR、NF-κB、MAPK、TNF、JAK-STAT和NOD样受体信号通路富集。不同KEGG通路中有几个DEG重叠，表明它们之间存在串扰。这些DEG之间预测的蛋白质-蛋白质相互作用表明，TLR4和CD14对多杀性巴氏杆菌LPS或外膜成分的识别，通过MyD88、IRAKs和/或TRAF6导致细胞内信号传导，进而激活NFκB和MAPK通路及相关细胞因子。
