Geipel U, Kropf J, Krause W, Gressner A M
Department of Clinical Chemistry, Philipps-University, Marburg, Germany.
Andrologia. 1992 Jul-Aug;24(4):205-11. doi: 10.1111/j.1439-0272.1992.tb02638.x.
The extracellular matrix components laminin, N-terminal propeptide of type III procollagen (PIIINP) and hyaluronan (HA) were determined in seminal fluids of 119 patients submitted for diagnosis of infertility. The concentrations of laminin and HA, but not those of PIIINP, were elevated in seminal fluid in comparison to their ranges of concentration in normal sera. Only weak correlations were observed between the concentrations of the three matrix components. The concentration of HA was negatively correlated with sperm count and ejaculate volume. Laminin was positively correlated with sperm count, the age of patients, and highly significantly with the concentrations of acrosin. A highly significant positive correlation was also found between PIIINP and fructose. By analysis of variance it could be shown that patients with azoospermia and oligozoospermia have significantly higher levels of HA than those with normospermia. Patients with terato- and asthenozoospermia showed no characteristic pattern of the matrix components.
对119例因不育前来诊断的患者的精液进行了检测，测定了细胞外基质成分层粘连蛋白、III型前胶原N端前肽（PIIINP）和透明质酸（HA）。与正常血清中的浓度范围相比，精液中层粘连蛋白和HA的浓度升高，但PIIINP的浓度未升高。三种基质成分的浓度之间仅观察到微弱的相关性。HA的浓度与精子计数和射精量呈负相关。层粘连蛋白与精子计数、患者年龄呈正相关，与顶体蛋白酶的浓度呈高度显著正相关。PIIINP与果糖之间也发现高度显著的正相关。通过方差分析表明，无精子症和少精子症患者的HA水平显著高于正常精子症患者。畸形精子症和弱精子症患者未表现出基质成分的特征性模式。
