Liao Fei, Zhu Xiao-yun, Wang Yong-mei, Zhao Yun-sheng, Zhu Lian-ping, Zuo Yu-ping
Laboratory of Bioinformatics & Molecular Engineering, Chongqing Key Laboratory of Biochemistry & Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University, Chongqing 400016, China.
J Zhejiang Univ Sci B. 2007 Apr;8(4):237-41. doi: 10.1631/jzus.2007.B0237.
The correlation of serum arylesterase (PON1) activity on phenylacetate determined by an integrated method to classical biochemical indexes of liver damage was investigated for the use of PON1 activity to evaluate liver damage. PON1 reaction curve as absorbance at 270 nm for 0.20 mmol/L phenylacetate hydrolysis was analyzed by the integrated method to determine maximal PON1 reaction rate. Classical biochemical indexes of liver damage were determined routinely. The 95% confidence threshold of PON1 activity in sera from healthy individuals was 2.12 mkat/L [(4.73+/-1.31) mkat/L, n=105]. PON1 activity in clinical sera was closely correlated to serum albumin, total protein and the ratio of albumin to globulins, but was weakly correlated to both direct and total bilirubin in serum. There were no correlations of PON1 activity to gamma-glutamyltransferase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase. Among 127 clinical sera with PON1 activity>2.12 mkat/L, there were 92% healthy individuals examined by albumin, 90% healthy individuals examined by total protein, 88% healthy individuals examined by total bilirubin, 86% healthy individuals examined by direct bilirubin and 64% healthy individuals examined by the ratio of albumin to globulins, respectively. In each group of healthy individuals judged by classical biochemical indexes of close correlation to PON1 activity, percentage of healthy individuals examined by PON1 activity was always >80%. These results suggested PON1 activity on phenylacetate estimated by the integrated method was also suitable for the evaluation of liver damage.
为了利用血清芳香酯酶（PON1）活性评估肝损伤，采用综合法研究了血清中苯乙酸酯酶（PON1）活性与肝损伤经典生化指标之间的相关性。采用综合法分析0.20 mmol/L苯乙酸水解时在270 nm处的吸光度作为PON1反应曲线，以确定最大PON1反应速率。常规测定肝损伤的经典生化指标。健康个体血清中PON1活性的95%置信阈值为2.12 mkat/L[（4.73±1.31）mkat/L，n = 105]。临床血清中的PON1活性与血清白蛋白、总蛋白及白蛋白与球蛋白比值密切相关，但与血清直接胆红素和总胆红素均呈弱相关。PON1活性与γ-谷氨酰转移酶、碱性磷酸酶、丙氨酸氨基转移酶和天冬氨酸氨基转移酶均无相关性。在127例PON1活性>2.12 mkat/L的临床血清中，分别有92%的个体通过白蛋白检测为健康个体，90%通过总蛋白检测为健康个体，88%通过总胆红素检测为健康个体，86%通过直接胆红素检测为健康个体，64%通过白蛋白与球蛋白比值检测为健康个体。在根据与PON1活性密切相关的经典生化指标判断的每组健康个体中，通过PON1活性检测的健康个体百分比均>80%。这些结果表明，采用综合法测定的血清苯乙酸酯酶（PON1）活性也适用于肝损伤的评估。
