细胞黏菌趋化性中叶酸分子活性部分的测定。
Determination of the active portion of the folic acid molecule in cellular slime mold chemotaxis.
作者信息
Pan P, Hall E M, Bonner J T
出版信息
J Bacteriol. 1975 Apr;122(1):185-91. doi: 10.1128/jb.122.1.185-191.1975.
Abstract
From earlier work it is known that folic acid attracts the amoebae of various species of cellular slime molds (11). Here we have tested a wide variety of pteridines, pyrimidines, and pyrazines to determine what part of the folic acid molecule is chemotactically active. It was shown that the activity lies in the pteridine ring itself. Furthermore, the cell-free supernatants of slime mold amoebae contain an enzyme that renders pterin and folic acid chemotactically inactive, which apparently increases the chemotactic sensitivity of the amoebae to those compounds. Despite the fact that slime mold amoebae secrete small amounts of folic acid-related compounds, there is no evidence that folates are acrasins; rather it is postulated that attraction to folates may be a food-seeking device for the amoebae which prey on folate-secreting bacteria in the soil.
摘要
从早期的研究工作中可知，叶酸能吸引各种细胞黏菌的变形虫（11）。在此，我们测试了多种蝶啶、嘧啶和吡嗪，以确定叶酸分子的哪一部分具有趋化活性。结果表明，活性存在于蝶啶环本身。此外，黏菌变形虫的无细胞上清液中含有一种酶，该酶会使蝶呤和叶酸失去趋化活性，这显然提高了变形虫对这些化合物的趋化敏感性。尽管黏菌变形虫会分泌少量与叶酸相关的化合物，但没有证据表明叶酸是聚集素；相反，据推测，对叶酸的吸引可能是变形虫寻找食物的一种方式，因为它们以土壤中分泌叶酸的细菌为食。
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