Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang, Malaysia.
Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Hibikino, Wakamatsu, Kitakyushu, Fukuoka, Japan.
PLoS One. 2018 Jun 26;13(6):e0199742. doi: 10.1371/journal.pone.0199742. eCollection 2018.
In order to clarify the in vitro cytotoxicity effect of superheated steam (SHS) treated poly((R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate) (PHBHHx) for biomaterial applications, SHS-treated PHBHHx oligoester samples: P(HB-co-6%-HHx) and P(HB-co-11%-HHx) with low and high percentages of unsaturated chain ends were evaluated for their cytotoxicity effects toward the growth of mouse fibroblast cell line NIH 3T3. From the results obtained after 24 and 48 h of the growth test, the SHS-treated PHBHHx oligoesters were found to be nontoxic to the growth of mouse fibroblast NIH 3T3 cell line with cell viability percentages of more than 95%. In order to serve as a potential resorbable medical suture, PHBHHx oligoesters were blended with poly(L-lactic acid) (PLLA) with a weight ratio of PHBHHx oligoester/PLLA = 20:80 (wt/wt) to improve mechanical properties of PHBHHx oligoesters. The PHBHHx oligoesters/PLLA blend films were evaluated for their thermal, mechanical, and surface wetting properties. Thermal properties of the blend films suggested a good compatibility between PHBHHx oligoesters and PLLA components. Mechanical properties of the blend films were determined to be close enough to a desirable strength range of medical sutures. Moreover, contact angle range of 65 < θ < 70° for the blend samples could provide desirable cell adhesion when used as biomaterials. Therefore, the blend of SHS-treated PHBHHx oligoesters and PLLA would be an ideal choice to be used as biomedical materials.
为了阐明超热蒸汽（SHS）处理的聚（R）-3-羟基丁酸酯-共-（R）-3-羟基己酸酯（PHBHHx）在生物材料应用中的体外细胞毒性作用，对低和高不饱和链末端百分比的 SHS 处理的 PHBHHx 低聚物样品：P（HB-co-6%-HHx）和 P（HB-co-11%-HHx）进行了细胞毒性评估对小鼠成纤维细胞 NIH 3T3 细胞系的生长。从生长试验 24 和 48 小时后的结果来看，发现 SHS 处理的 PHBHHx 低聚物对小鼠成纤维细胞 NIH 3T3 细胞系的生长没有毒性，细胞活力百分比超过 95%。为了用作潜在的可吸收医用缝线，将 PHBHHx 低聚物与聚（L-乳酸）（PLLA）以 PHBHHx 低聚物/PLLA = 20:80（wt/wt）的重量比混合，以改善 PHBHHx 低聚物的机械性能。评估了 PHBHHx 低聚物/PLLA 共混膜的热、机械和表面润湿性能。共混膜的热性能表明 PHBHHx 低聚物和 PLLA 成分之间具有良好的相容性。共混膜的力学性能被确定为接近医用缝线所需的理想强度范围。此外，共混样品的接触角范围为 65 <θ<70°，用作生物材料时可提供理想的细胞黏附性。因此，SHS 处理的 PHBHHx 低聚物和 PLLA 的混合物将是用作生物医学材料的理想选择。
