Hara-Kud Yukiko, Miwa Norinaga, Yamasaki Syougo, Yatsuyanagi Jun, Iwade Yoshito, Takahash Hajime, Miyasaka Jiro
Division of Microbiology, National Institute of Health Sciences.
Kansenshogaku Zasshi. 2005 Dec;79(12):931-6. doi: 10.11150/kansenshogakuzasshi1970.79.931.
To quantify the number of Vibrio vulnificus in shellfish, we compared the most probable number (MPN) combined with a culture (MPN-culture) or polymerase-chain reaction (PCR) assay (MPN-PCR) to a quantitative PCR assay. Enrichment in alkaline peptone water by MPN was conducted at 25 and 35 degrees C. Enrichment at 35 degrees C was superior or similar to enrichment at 25 degrees C in over 65% of samples by MPNculture and in more than 75% of samples by MPN-PCR assay. V. vulnificus was more easily isolated on chromogenic agar medium during culture, MPN-PCR assay was superior or similar to MPNculture in over 90% of samples by enrichment at 25 degrees C and to over 88% of samples by enrichment at 35 degrees C. The number of V. vulnificus by quantitative PCR assay was similar to that of MPN-PCR assay in 6 of 8 samples but not from MPNculture. V. vulnificus contamination was frequently detected in samples from Kyushu Island.
为了量化贝类中创伤弧菌的数量，我们将最可能数（MPN）结合培养法（MPN-培养法）或聚合酶链反应（PCR）检测法（MPN-PCR）与定量PCR检测法进行了比较。通过MPN在碱性蛋白胨水中于25℃和35℃进行增菌培养。在超过65%的样本中，35℃增菌培养的效果在MPN-培养法中优于或类似于25℃增菌培养，在超过75%的样本中，35℃增菌培养的效果在MPN-PCR检测法中也是如此。在培养过程中，创伤弧菌在显色琼脂培养基上更容易分离，在25℃增菌培养的超过90%的样本以及35℃增菌培养的超过88%的样本中，MPN-PCR检测法优于或类似于MPN-培养法。在8个样本中的6个样本中，定量PCR检测法得出的创伤弧菌数量与MPN-PCR检测法相似，但与MPN-培养法得出的数量不同。在九州岛采集的样本中经常检测到创伤弧菌污染。
