Ogilvie A C, Groeneveld A B, Straub J P, Thijs L G
Medical Intensive Care Unit, Free University Hospital, The Netherlands.
Intensive Care Med. 1991;17(1):40-4. doi: 10.1007/BF01708408.
In order to assess if an oxidant/antioxidant imbalance is involved in human septic shock and its outcome, we measured plasma levels of the lipid peroxides malondialdehyde--as thiobarbituric acid reactive substance--conjugated dienes and fluorescent products, together with the antioxidants alpha-tocopherol, glutathione peroxidase activity and selenium in 12 patients with septic shock and compared them with values of normal controls. At first measurements, malondialdehyde (median 3.9 mumol/l; range 2-38.8) and fluorescent products (median 21.2%; range 9.4-134) were elevated (p less than 0.05), alpha-tocopherol (median 15 mumol/l; range 7-25) and selenium (median 0.76 micrograms/ml; range 0.49-1.09) were depressed (p less than 0.05). Conjugated dienes and glutathione peroxidase activity were in the normal range. In non-survivors (n = 5) initial levels of malondialdehyde and fluorescent products (median 11 versus 3.1 mumol/l; 74 versus 13% respectively) were higher than in survivors (p less than 0.05) and initial selenium levels were lower (median 0.58 versus 0.92 micrograms/l; p less than 0.05). These results are consistent with the concept that an oxidant/antioxidant imbalance--as indicated by elevated plasma lipid peroxides and depressed antioxidants--is involved in human septic shock and a fatal outcome.
为了评估氧化/抗氧化失衡是否与人类脓毒症休克及其预后有关，我们检测了12例脓毒症休克患者血浆中脂质过氧化物丙二醛（作为硫代巴比妥酸反应物质）、共轭二烯和荧光产物的水平，以及抗氧化剂α-生育酚、谷胱甘肽过氧化物酶活性和硒的水平，并将其与正常对照组的值进行比较。在首次检测时，丙二醛（中位数3.9μmol/L；范围2 - 38.8）和荧光产物（中位数21.2%；范围9.4 - 134）升高（p < 0.05），α-生育酚（中位数15μmol/L；范围7 - 25）和硒（中位数0.76μg/ml；范围0.49 - 1.09）降低（p < 0.05）。共轭二烯和谷胱甘肽过氧化物酶活性在正常范围内。在非存活者（n = 5）中，丙二醛和荧光产物的初始水平（分别为中位数11对3.1μmol/L；74对13%）高于存活者（p < 0.05），初始硒水平较低（中位数0.58对0.92μg/L；p < 0.05）。这些结果与以下概念一致，即氧化/抗氧化失衡（如血浆脂质过氧化物升高和抗氧化剂降低所示）与人类脓毒症休克及致命预后有关。
