Hirt L, Hirsch-Behnam A, de Villiers E M
Referenzzentrum fuer humanpathogene Papillomviren, Deutsches Krebsforschungszentrum, Heidelberg, F.R.G.
Virus Res. 1991 Mar;18(2-3):179-89. doi: 10.1016/0168-1702(91)90017-p.
The complete nucleotide sequence of human papillomavirus type 41 (HPV-41) has been determined. HPV-41 was originally isolated from a facial wart, but its DNA has subsequently been detected in some skin carcinomas and premalignant keratoses (Grimmel et al., Int. J. Cancer, 1988, 41, 5-9; de Villiers, Grimmel and Neumann, unpublished results). The analysis of the cloned HPV-41 nucleic acid reveals that its genome organisation is characteristic as for other papillomavirus types. Yet, the analysis indicates at the same time that this virus is most distantly related to all other types of human-pathogenic papillomaviruses sequenced thus far and appears to identify HPV-41 as the first member of a new subgroup of HPV. The overall nucleotide homology to other sequenced HPV types is below 50%. The closest other HPV type is represented by HPV-18, sharing 49% identical nucleotides. The typical E2 binding sequence ACCN6GGT, found in all papillomaviruses analyzed to date, does not occur in the URR of the HPV-41 genome. Modified E2 binding sequences, as described for BPV 1 (Li et al., Genes Dev. 1989, 3, 510-526), are located in the domain proximal to the E6 ORF. These are ACCN6GTT, AACN6GGT and the two perfect palindromic sequences AACGAATTCGTT.
人乳头瘤病毒41型（HPV - 41）的完整核苷酸序列已被确定。HPV - 41最初是从面部疣中分离出来的，但随后在一些皮肤癌和癌前角化病中检测到了它的DNA（Grimmel等人，《国际癌症杂志》，1988年，41卷，5 - 9页；de Villiers、Grimmel和Neumann，未发表结果）。对克隆的HPV - 41核酸的分析表明，其基因组结构与其他乳头瘤病毒类型具有特征性。然而，分析同时表明，该病毒与迄今为止测序的所有其他人类致病性乳头瘤病毒类型的亲缘关系最为疏远，似乎将HPV - 41鉴定为HPV一个新亚组的首个成员。与其他已测序的HPV类型的总体核苷酸同源性低于50%。最接近的其他HPV类型是HPV - 18，共享49%的相同核苷酸。在迄今为止分析的所有乳头瘤病毒中都发现的典型E2结合序列ACCN6GGT，在HPV - 41基因组的上游调控区（URR）中不存在。如BPV 1所述的修饰E2结合序列（Li等人，《基因与发育》，1989年，3卷，510 - 526页），位于靠近E6开放阅读框的结构域中。这些序列是ACCN6GTT、AACN6GGT以及两个完美的回文序列AACGAATTCGTT。
