Kinami Y, Ashida Y, Seto K, Takashima S, Kita I
Department of Surgery II, Kanazawa Medical University, Ishikawa, Japan.
HPB Surg. 1991 Feb;3(2):117-25; discussion 125-7. doi: 10.1155/1991/65315.
This study was carried out to clarify the influence of incomplete bile duct obstruction (IBDO) on the occurrence and proliferation of cholangiocarcinoma and to evaluate the effect of release of IBDO at an early stage, using 175 Syrian golden hamsters. These hamsters received 500 mg/kg body weight of diisopropanolnitrosamine (DIPN) once weekly for 10 weeks, and then were divided into 3 groups, consisting of the simple laparotomy group (SL group), the IBDO group and 2 week IBDO group, in which IBDO was released after 2 weeks. The occurrence rates of cholangiocarcinoma at 20 weeks were 42% in the SL group, 76% in the IBDO group and 30% in the 2 week IBDO group. The mean numbers of tumors per hamster in the IBDO group were significantly greater than those in other groups (p less than 0.05). Both occurrence rates and numbers of tumors in the 2 week IBDO group were similar to those in the SL group. The proliferation of bile ductules and isolation of bacteria from bile in the IBDO group had higher rates at 15, 20 weeks than those found in the other groups. These results suggest that IBDO has an influence, as promoter, on the occurrence of cholangiocarcinoma induced by DIPN, and the disappearance of its promoting effect is caused by release of the obstruction.
本研究旨在阐明不完全胆管梗阻（IBDO）对胆管癌发生和增殖的影响，并评估早期解除IBDO的效果，使用了175只叙利亚金黄地鼠。这些地鼠每周一次接受500mg/kg体重的二异丙基亚硝胺（DIPN），共10周，然后分为3组，包括单纯剖腹手术组（SL组）、IBDO组和2周IBDO组，其中2周IBDO组在2周后解除梗阻。20周时胆管癌的发生率在SL组为42%，在IBDO组为76%，在2周IBDO组为30%。IBDO组每只地鼠的平均肿瘤数显著多于其他组（p小于0.05）。2周IBDO组的发生率和肿瘤数与SL组相似。IBDO组在15、20周时胆管小分支的增殖和胆汁中细菌的分离率高于其他组。这些结果表明，IBDO作为促进剂对DIPN诱导的胆管癌发生有影响，其促进作用的消失是由梗阻解除所致。
