Kinami Y, Ashida Y, Gotoda H, Seto K, Kojima Y, Takashima S
Division of Cancer Research, Kanazawa Medical University, Ishikawa, Japan.
Oncology. 1993;50(1):46-51. doi: 10.1159/000227146.
This study was performed to clarify the promoting effects of primary or secondary bile acid load on the occurrence of cholangiocarcinoma, using Syrian golden hamsters. These hamsters received subcutaneously diisopropanolnitrosamine (DIPN) once weekly for 10 weeks, and simultaneously were given a standard pellet diet (control group) containing taurocholic acid (TCA group) or deoxycholic acid (DCA group). The rates of cholangiocarcinoma at 20 weeks were 23% in the control group, 60% in the TCA group and 59% in the DCA group. There were significant differences between the control and the TCA or DCA groups (p < 0.05). The rates of proliferation of bile ductules or hyperplasia of the bile duct epithelium and the bromodeoxyuridine labeling indices of bile duct epithelial cells were high in both groups treated with bile acids, compared with those in the control group. Regarding the composition of bile acids in the intraductal bile, the TCA and DCA groups revealed a decrease in primary bile acids and an increase in DCA. These results suggest that both TCA and DCA given orally promote the occurrence of DIPN-induced cholangiocarcinoma.
本研究旨在利用叙利亚金黄地鼠阐明原发性或继发性胆汁酸负荷对胆管癌发生的促进作用。这些仓鼠每周皮下注射一次二异丙基亚硝胺（DIPN），持续10周，同时给予含牛磺胆酸的标准颗粒饲料（对照组）、牛磺胆酸（TCA组）或脱氧胆酸（DCA组）。20周时，对照组胆管癌发生率为23%，TCA组为60%，DCA组为59%。对照组与TCA组或DCA组之间存在显著差异（p<0.05）。与对照组相比，胆汁酸处理的两组胆管小分支增殖率、胆管上皮增生率及胆管上皮细胞溴脱氧尿苷标记指数均较高。关于导管内胆汁中胆汁酸的组成，TCA组和DCA组原发性胆汁酸减少，脱氧胆酸增加。这些结果表明，口服牛磺胆酸和脱氧胆酸均能促进DIPN诱导的胆管癌的发生。
