微流控芯片集成微腔表面等离子体共振传感器：葡萄糖氧化酶结合活性检测。
Microfluidic devices integrating microcavity surface-plasmon-resonance sensors: glucose oxidase binding-activity detection.
机构信息
Biocomplexity Institute, Department of Physics, Indiana University, Bloomington, Indiana 47405-7102, USA.
出版信息
Anal Chem. 2010 Jan 1;82(1):343-52. doi: 10.1021/ac902038d.
We have developed miniature (approximately 1 microm diameter) microcavity surface-plasmon-resonance sensors (MSPRS), integrated them with microfluidics, and tested their sensitivity to refractive-index changes. We tested their biosensing capability by distinguishing the interaction of glucose oxidase (M(r) 160 kDa) with its natural substrate (beta-D-glucose, M(r) 180 Da) from its interactions with nonspecific substrates (L-glucose, D-mannose, and 2-deoxy-D-glucose). We ran the identical protocol we had used with the MSPRS on a Biacore 3000 instrument using their bare gold chip. Only the MSPRS was able to detect beta-D-glucose binding to glucose oxidase. Each MSPRS can detect the binding to its surface of fewer than 35,000 glucose oxidase molecules (representing 9.6 fg or 60 zmol of protein), about 10(6) times fewer than classical surface-plasmon-resonance biosensors.
我们已经开发出了微型（直径约 1 微米）微腔表面等离子体共振传感器（MSPRS），并将其与微流控技术集成在一起，测试了它们对折射率变化的灵敏度。我们通过区分葡萄糖氧化酶（Mr 160 kDa）与其天然底物（β-D-葡萄糖，Mr 180 Da）之间的相互作用以及与非特异性底物（L-葡萄糖、D-甘露糖和 2-脱氧-D-葡萄糖）之间的相互作用来测试其生物传感能力。我们使用 Biacore 3000 仪器上的裸金芯片运行了与 MSPRS 相同的方案。只有 MSPRS 能够检测到β-D-葡萄糖与葡萄糖氧化酶的结合。每个 MSPRS 都可以检测到其表面结合的葡萄糖氧化酶分子少于 35000 个（代表 9.6 fg 或 60 zmol 蛋白质），大约比传统的表面等离子体共振生物传感器少 10^6 倍。
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