Klebanoff S J, Hillier S L, Eschenbach D A, Waltersdorph A M
Department of Medicine, University of Washington School of Medicine, Seattle.
J Infect Dis. 1991 Jul;164(1):94-100. doi: 10.1093/infdis/164.1.94.
H2O2-generating lactobacilli (LB+) are present in the vagina of most normal women but are absent from most women with bacterial vaginosis (BV). LB+ at high concentration was toxic to Gardnerella vaginalis (the predominant organism in the vagina of women with BV); when the LB+ was lowered to a level where it was ineffective alone, the addition of myeloperoxidase and chloride reinstituted toxicity. Toxicity was inhibited by catalase and was not seen when H2O2-negative lactobacilli were used, implicating H2O2 as the toxic molecule. LB+ could be replaced by H2O2 and chloride by iodide, bromide, or thiocyanate. The optimum pH for inhibition of G. vaginalis was 5.0-6.0 LB+ also was autoinhibitory when combined with myeloperoxidase and chloride. LB+ alone at low concentrations was toxic to Bacteroides bivius through the formation of H2O2. Adequate amounts of peroxidase were found in the vagina of 17 of 21 women. These findings suggest that LB+ may contribute to the control of the vaginal flora, particularly in the presence of peroxidase and a halide.
产生过氧化氢的乳酸杆菌（LB+）存在于大多数正常女性的阴道中，但大多数细菌性阴道病（BV）患者的阴道中则没有。高浓度的LB+对阴道加德纳菌（BV患者阴道中的主要微生物）有毒性；当LB+降至单独作用无效的水平时，添加髓过氧化物酶和氯离子可恢复毒性。过氧化氢酶可抑制毒性，使用过氧化氢阴性的乳酸杆菌时则未观察到毒性，这表明过氧化氢是有毒分子。LB+可用过氧化氢替代，氯离子可用碘化物、溴化物或硫氰酸盐替代。抑制阴道加德纳菌的最适pH值为5.0 - 6.0。当与髓过氧化物酶和氯离子结合时，LB+也具有自抑制作用。低浓度的LB+单独作用时，通过形成过氧化氢对双栖拟杆菌有毒性。21名女性中有17名的阴道中发现了足够量的过氧化物酶。这些发现表明，LB+可能有助于控制阴道菌群，尤其是在存在过氧化物酶和卤化物的情况下。
