Eschenbach D A, Davick P R, Williams B L, Klebanoff S J, Young-Smith K, Critchlow C M, Holmes K K
Department of Microbiology, University of Washington, Seattle 98195.
J Clin Microbiol. 1989 Feb;27(2):251-6. doi: 10.1128/jcm.27.2.251-256.1989.
A predominance of Lactobacillus species in the vaginal flora is considered normal. In women with bacterial vaginosis, the prevalence and concentrations of intravaginal Gardnerella vaginalis and anaerobes are increased, whereas the prevalence of intravaginal Lactobacillus species is decreased. Because some lactobacilli are known to produce hydrogen peroxide (H2O2), which can be toxic to organisms that produce little or no H2O2-scavenging enzymes (e.g., catalase), we postulated that an absence of H2O2-producing Lactobacillus species could allow an overgrowth of catalase-negative organisms, such as those found among women with bacterial vaginosis. In this study, H2O2-producing facultative Lactobacillus species were found in the vaginas of 27 (96%) of 28 normal women and 4 (6%) of 67 women with bacterial vaginosis (P less than 0.001). Anaerobic Lactobacillus species (which do not produce hydrogen peroxide) were isolated from 24 (36%) of 67 women with bacterial vaginosis and 1 (4%) of 28 normal women (P less than 0.001). The production of H2O2 by Lactobacillus species may represent a nonspecific antimicrobial defense mechanism of the normal vaginal ecosystem.
阴道菌群中乳酸杆菌占优势被认为是正常的。在患有细菌性阴道病的女性中，阴道内加德纳菌和厌氧菌的患病率及浓度会增加，而阴道内乳酸杆菌的患病率则会降低。由于已知一些乳酸杆菌会产生过氧化氢（H2O2），而过氧化氢对那些几乎不产生或不产生H2O2清除酶（如过氧化氢酶）的生物体可能有毒性，因此我们推测，缺乏产生H2O2的乳酸杆菌可能会使过氧化氢酶阴性的生物体过度生长，比如在患有细菌性阴道病的女性中发现的那些生物体。在本研究中，28名正常女性中有27名（96%）阴道内发现了能产生H2O2的兼性乳酸杆菌，67名患有细菌性阴道病的女性中有4名（6%）阴道内发现了能产生H2O2的兼性乳酸杆菌（P<0.001）。67名患有细菌性阴道病的女性中有24名（36%）分离出了不产生过氧化氢的厌氧乳酸杆菌，28名正常女性中有1名（4%）分离出了不产生过氧化氢的厌氧乳酸杆菌（P<0.001）。乳酸杆菌产生H2O2可能代表了正常阴道生态系统的一种非特异性抗菌防御机制。
