Czeglédy J, Evander M, Veres L, Gergely L, Wadell G
Institute of Microbiology University Medical School of Debrecen, Hungary.
Med Microbiol Immunol. 1991;180(1):37-43. doi: 10.1007/BF00191699.
In a study of 29 cases of histologically confirmed, characterized colposcopically and cytologically, cervical intraepithelial neoplasias 48.3% (14/29) of biopsies were positive for human papillomavirus (HPV) type 16 DNA by polymerase chain reaction. We used two oligonucleotide primer pairs (position 215-514 and 606-805) flanking a 300 and a 199 base pair fragment from the early 6 (E6) and early 7 (E7) genes. The results were concordant both with the E6 and with the E7 regions. Of the amplified products 85.7% (12/14) could be confirmed; these carried 16 specific sequences by Southern blot hybridization. HPV 16 DNA was present in 6.7% (2/30) of the colposcopically directed cytologically normal matched control samples using the same methods.
在一项对29例经组织学确诊、阴道镜检查和细胞学特征明确的宫颈上皮内瘤变的研究中，通过聚合酶链反应，48.3%（14/29）的活检标本人乳头瘤病毒（HPV）16型DNA呈阳性。我们使用了两对寡核苷酸引物对（位置215 - 514和606 - 805），分别位于来自早期6（E6）和早期7（E7）基因的300和199个碱基对片段两侧。结果在E6和E7区域均一致。在扩增产物中，85.7%（12/14）可得到确认；通过Southern印迹杂交，这些产物带有16种特异性序列。使用相同方法，在阴道镜引导下细胞学正常的匹配对照样本中，6.7%（2/30）存在HPV 16 DNA。
