探索真核生物中的内体分选转运复合体（ESCRT）机制
Exploring the ESCRTing machinery in eukaryotes.
作者信息
Winter Verena, Hauser Marie-Theres
机构信息
Institute of Applied Genetics and Cell Biology, Department of Applied Plant Sciences and Plant Biotechnology, BOKU - University of Natural Resources and Applied Life Sciences, Vienna, Austria.
出版信息
Trends Plant Sci. 2006 Mar;11(3):115-23. doi: 10.1016/j.tplants.2006.01.008. Epub 2006 Feb 20.
Abstract
The profile of protein sorting into multivesicular bodies (MVBs) has risen recently with the identification of three heteromeric complexes known as ESCRT-I,-II,-III (Endosomal Sorting Complex Required for Transport). Genetic analyses in yeast have identified up to 15 soluble class E VPS (vacuolar protein sorting) proteins that have been assigned to the ESCRT machinery and function in cargo recognition and sorting, complex assembly, vesicle formation and dissociation. Despite their functional importance in yeast and mammalian cells, little is known about their presence and function in other organisms including plants. We have made use of the fully sequenced genomes of Arabidopsis thaliana and Oryza sativa, Drosophila melanogaster and Caenorhabditis elegans to explore the identity, structural characteristics and phylogenetic relationships of proteins assigned to the ESCRT machinery.
摘要
随着三种被称为ESCRT-I、-II、-III（运输所需的内体分选复合体）的异源复合体的鉴定，蛋白质分选进入多泡体（MVBs）的研究最近受到了更多关注。酵母中的遗传分析已经鉴定出多达15种可溶性E类VPS（液泡蛋白分选）蛋白，这些蛋白已被归入ESCRT机制，并在货物识别与分选、复合体组装、囊泡形成和解离中发挥作用。尽管它们在酵母和哺乳动物细胞中具有重要功能，但对于它们在包括植物在内的其他生物体中的存在和功能却知之甚少。我们利用拟南芥、水稻、黑腹果蝇和秀丽隐杆线虫的全基因组序列，来探索归入ESCRT机制的蛋白质的身份、结构特征和系统发育关系。
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