Gelderblom W C, Kriek N P, Marasas W F, Thiel P G
Research Institute for Nutritional Diseases, South African Medical Research Council, Tygerberg.
Carcinogenesis. 1991 Jul;12(7):1247-51. doi: 10.1093/carcin/12.7.1247.
A semi-purified corn-based diet containing 50 mg/kg of pure (not less than 90%) fumonisin B1 (FB1), isolated from culture material of Fusarium moniliforme strain MRC 826, was fed to a group of 25 rats over a period of 26 months. A control group of 25 rats received the same diet without FB1. Five rats from each group were killed at 6, 12, 20 and 26 months. The liver was the main target organ in the FB1-treated rats and the hepatic pathological changes were identical to those previously reported in rats fed culture material of F.moniliforme MRC 826. All FB1-treated rats that died or were killed from 18 months onwards suffered from a micro- and macronodular cirrhosis and had large expansile nodules of cholangiofibrosis at the hilus of the liver. Ten out of 15 FB1-treated rats (66%) that were killed and/or died between 18 and 26 months developed primary hepatocellular carcinoma. Metastases to the heart, lungs or kidneys were present in four of the rats with hepatocellular carcinoma. No neoplastic changes were observed in any of the control rats. Chronic interstitial nephritis was present in the kidneys of FB1-treated rats killed after 26 months. No lesions were observed in the esophagus, heart or forestomach of FB1-treated rats and this is contrary to previous findings when culture material of the fungus was fed to rats. It is concluded that FB1 is responsible for the hepatocarcinogenic and the hepatotoxic but not all the other toxic effects of culture material of F.moniliforme MRC 826 in rats.
给一组25只大鼠喂食一种半纯化的玉米基日粮，该日粮含有从镰刀菌MRC 826菌株培养物中分离出的50毫克/千克纯（纯度不低于90%）伏马菌素B1（FB1），持续26个月。另一组25只大鼠作为对照组，喂食不含FB1的相同日粮。每组分别在6个月、12个月、20个月和26个月时处死5只大鼠。肝脏是FB1处理组大鼠的主要靶器官，肝脏病理变化与先前喂食镰刀菌MRC 826培养物的大鼠所报告的变化相同。从18个月起死亡或被处死的所有FB1处理组大鼠均患有小结节性和大结节性肝硬化，且在肝门处有大的扩张性胆管纤维化结节。在18至26个月期间被处死和/或死亡的15只FB1处理组大鼠中，有10只（66%）发生了原发性肝细胞癌。患有肝细胞癌的4只大鼠出现了转移至心脏、肺或肾脏的情况。在任何对照组大鼠中均未观察到肿瘤性变化。在26个月后处死的FB1处理组大鼠的肾脏中存在慢性间质性肾炎。在FB1处理组大鼠的食管、心脏或前胃中未观察到病变，这与先前将该真菌培养物喂食大鼠时的发现相反。得出的结论是，FB1是导致大鼠肝细胞癌和肝毒性的原因，但不是镰刀菌MRC 826培养物对大鼠的所有其他毒性作用的原因。
