Honda Masaki J, Tsuchiya Shuhei, Sumita Yoshinori, Sagara Hiroshi, Ueda Minoru
Tooth Regeneration, Division of Stem Cell Engineering, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Biomaterials. 2007 Feb;28(4):680-9. doi: 10.1016/j.biomaterials.2006.09.039. Epub 2006 Oct 12.
Progress is being made toward regenerating teeth by seeding dissociated postnatal odontogenic cells onto scaffolds and implanting them in vivo, but tooth morphology remains difficult to control. In this study, we aimed to facilitate tooth regeneration using a novel technique to sequentially seed epithelial cells and mesenchymal cells so that they formed appropriate interactions in the scaffold. Dental epithelium and mesenchyme from porcine third molar teeth were enzymatically separated and dissociated into single cells. Mesenchymal cells were seeded onto the surface of the scaffold and epithelial cells were then plated on top so that the two cell types were in direct contact. The cell-scaffold constructs were evaluated in vitro and also implanted into immunocompromised rats for in vivo analysis. Control groups included constructs where direct contact between the two cell types was prevented. In scaffolds seed using the novel technique, alkaline phosphatase activity was significantly greater than controls, the tooth morphology in vivo was developed in similar to that of natural tooth, and only one tooth structure formed in each scaffold. These results suggest that the novel cell-seeding technique could be useful for regulating the morphology of regenerated teeth.
通过将出生后解离的牙源性细胞接种到支架上并将其植入体内来再生牙齿的研究正在取得进展，但牙齿形态仍然难以控制。在本研究中，我们旨在使用一种新技术促进牙齿再生，该技术可依次接种上皮细胞和间充质细胞，使它们在支架中形成适当的相互作用。从猪第三磨牙中酶解分离出牙上皮和牙间充质，并将其解离为单细胞。将间充质细胞接种到支架表面，然后在上层接种上皮细胞，使两种细胞类型直接接触。对细胞-支架构建体进行体外评估，并将其植入免疫缺陷大鼠体内进行体内分析。对照组包括阻止两种细胞类型直接接触的构建体。在使用新技术接种的支架中，碱性磷酸酶活性显著高于对照组，体内牙齿形态与天然牙齿相似，每个支架中仅形成一种牙齿结构。这些结果表明，这种新的细胞接种技术可能有助于调节再生牙齿的形态。
