使用植绒拭子和通用运输培养基增强沙眼衣原体和淋病奈瑟菌的分子检测。
Use of flocked swabs and a universal transport medium to enhance molecular detection of Chlamydia trachomatis and Neisseria gonorrhoeae.
作者信息
Chernesky Max, Castriciano Santina, Jang Dan, Smieja Marek
机构信息
McMaster University, St. Joseph's Healthcare and Father Sean O'Sullivan Research Centre, 50 Charlton Ave. East, Hamilton, ON L8N 4A6, Canada.
出版信息
J Clin Microbiol. 2006 Mar;44(3):1084-6. doi: 10.1128/JCM.44.3.1084-1086.2006.
Abstract
The use of new flocked swabs, compared to kit swabs, enhanced the ability of three commercial nucleic acid amplification tests to detect low levels of Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acids when the organisms were diluted in a universal transport medium as mocked specimens.
摘要
与试剂盒拭子相比，当将沙眼衣原体和淋病奈瑟菌在通用运输介质中作为模拟标本进行稀释时，新型植绒拭子的使用增强了三种商业核酸扩增检测方法检测低水平沙眼衣原体和淋病奈瑟菌核酸的能力。
相似文献
1
Use of flocked swabs and a universal transport medium to enhance molecular detection of Chlamydia trachomatis and Neisseria gonorrhoeae.使用植绒拭子和通用运输培养基增强沙眼衣原体和淋病奈瑟菌的分子检测。
J Clin Microbiol. 2006 Mar;44(3):1084-6. doi: 10.1128/JCM.44.3.1084-1086.2006.
2
Multicenter evaluation of the BDProbeTec ET System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs.BDProbeTec ET系统用于检测尿液标本、女性宫颈拭子和男性尿道拭子中沙眼衣原体和淋病奈瑟菌的多中心评估。
J Clin Microbiol. 2001 Mar;39(3):1008-16. doi: 10.1128/JCM.39.3.1008-1016.2001.
3
Validation of the APTIMA Combo 2 assay for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in SurePath liquid-based pap test samples taken with different collection devices.在使用不同采集装置采集的SurePath液基巴氏试验样本中，验证APTIMA Combo 2检测法用于检测沙眼衣原体和淋病奈瑟菌的效果。
Sex Transm Dis. 2009 Sep;36(9):581-3. doi: 10.1097/OLQ.0b013e3181a4c3fc.
4
5
Performance of strand displacement amplification assay in the detection of Chlamydia trachomatis and Neisseria gonorrhoeae.链置换扩增检测法在沙眼衣原体和淋病奈瑟菌检测中的性能
Jpn J Infect Dis. 2005 Oct;58(5):283-8.
6
Molecular genetic methods for diagnosis and characterisation of Chlamydia trachomatis and Neisseria gonorrhoeae: impact on epidemiological surveillance and interventions.沙眼衣原体和淋病奈瑟菌诊断及特征分析的分子遗传学方法：对流行病学监测和干预措施的影响
APMIS. 2004 Nov-Dec;112(11-12):771-84. doi: 10.1111/j.1600-0463.2004.apm11211-1205.x.
7
Ability of the digene hybrid capture II test to identify Chlamydia trachomatis and Neisseria gonorrhoeae in cervical specimens.Digene杂交捕获II试验在宫颈标本中鉴定沙眼衣原体和淋病奈瑟菌的能力。
J Clin Microbiol. 1999 Nov;37(11):3668-71. doi: 10.1128/JCM.37.11.3668-3671.1999.
8
9
Evaluation of an automated extraction method for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Cobas Amplicor PCR from different sample materials.评估一种通过Cobas Amplicor聚合酶链反应从不同样本材料中检测沙眼衣原体和淋病奈瑟菌的自动提取方法。
Scand J Infect Dis. 2007;39(5):405-8. doi: 10.1080/00365540601087582.
10
DNA sequencing validation of Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acid tests.沙眼衣原体和淋病奈瑟菌核酸检测的DNA测序验证
Am J Clin Pathol. 2008 Jun;129(6):852-9. doi: 10.1309/Y2202RVCGRWW1RWB.
引用本文的文献
1
Diagnostic armamentarium of infectious keratitis: A comprehensive review.感染性角膜炎的诊断方法学：全面综述。
Ocul Surf. 2022 Jan;23:27-39. doi: 10.1016/j.jtos.2021.11.003. Epub 2021 Nov 13.
2
Molecular Detection and Identification of in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain.西班牙北部野生和家养反刍动物宿主眼部的分子检测与鉴定
Pathogens. 2021 Mar 23;10(3):383. doi: 10.3390/pathogens10030383.
3
Reduction of non-typeable results using a plasmid oriented Lymfogranuloma venereum PCR for typing of Chlamydia trachomatis positive samples.采用质粒定向淋球菌 PCR 减少非定型结果，对沙眼衣原体阳性样本进行分型。
PLoS One. 2020 Jun 4;15(6):e0233990. doi: 10.1371/journal.pone.0233990. eCollection 2020.
4
FLOQSwab™: Optimisation of Procedures for the Recovery of Microbiological Samples from Surfaces.FLOQSwab™：从表面采集微生物样本的程序优化
Ital J Food Saf. 2016 Aug 3;5(3):5756. doi: 10.4081/ijfs.2016.5756. eCollection 2016 Jun 3.
5
Development and Validation of a Highly Accurate Quantitative Real-Time PCR Assay for Diagnosis of Bacterial Vaginosis.用于诊断细菌性阴道病的高准确度定量实时聚合酶链反应检测方法的开发与验证
J Clin Microbiol. 2016 Apr;54(4):1017-24. doi: 10.1128/JCM.03104-15. Epub 2016 Jan 27.
6
Streptococcus equi Detection Polymerase Chain Reaction Assay for Equine Nasopharyngeal and Guttural Pouch Wash Samples.用于马鼻咽和咽囊冲洗样本的马链球菌检测聚合酶链反应测定法
J Vet Intern Med. 2016 Jan-Feb;30(1):276-81. doi: 10.1111/jvim.13808. Epub 2015 Dec 17.
7
Randomized Cross-Sectional Study to Compare HIV-1 Specific Antibody and Cytokine Concentrations in Female Genital Secretions Obtained by Menstrual Cup and Cervicovaginal Lavage.比较月经杯和宫颈阴道灌洗获取的女性生殖道分泌物中HIV-1特异性抗体和细胞因子浓度的随机横断面研究。
PLoS One. 2015 Jul 6;10(7):e0131906. doi: 10.1371/journal.pone.0131906. eCollection 2015.
8
Successful Combination of Nucleic Acid Amplification Test Diagnostics and Targeted Deferred Neisseria gonorrhoeae Culture.核酸扩增检测诊断与靶向延迟淋病奈瑟菌培养的成功结合。
J Clin Microbiol. 2015 Jun;53(6):1884-90. doi: 10.1128/JCM.00369-15. Epub 2015 Apr 1.
9
Nasal screening for MRSA: different swabs--different results!耐甲氧西林金黄色葡萄球菌的鼻腔筛查：不同拭子——不同结果！
PLoS One. 2014 Oct 29;9(10):e111627. doi: 10.1371/journal.pone.0111627. eCollection 2014.
10
Evaluation of anatomically designed flocked rectal swabs for molecular detection of enteric pathogens in children admitted to hospital with severe gastroenteritis in Botswana.评价在博茨瓦纳因严重胃肠炎住院的儿童中，使用解剖设计的植绒直肠拭子进行肠道病原体分子检测的效果。
J Clin Microbiol. 2014 Nov;52(11):3922-7. doi: 10.1128/JCM.01894-14. Epub 2014 Aug 27.
本文引用的文献
1
Confirming positive results of nucleic acid amplification tests (NAATs) for Chlamydia trachomatis: all NAATs are not created equal.确认沙眼衣原体核酸扩增检测（NAATs）的阳性结果：并非所有NAATs都一样。
J Clin Microbiol. 2005 Mar;43(3):1372-3. doi: 10.1128/JCM.43.3.1372-1373.2005.
2
Comparison of the APTIMA CT and GC assays with the APTIMA combo 2 assay, the Abbott LCx assay, and direct fluorescent-antibody and culture assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae.将APTIMA CT和GC检测法与APTIMA combo 2检测法、雅培LCx检测法以及直接荧光抗体检测法和培养检测法进行比较，以检测沙眼衣原体和淋病奈瑟菌。
J Clin Microbiol. 2004 Jul;42(7):3089-93. doi: 10.1128/JCM.42.7.3089-3093.2004.
3
Comparison of three nucleic acid amplification tests for detection of Chlamydia trachomatis in urine specimens.三种核酸扩增试验检测尿液标本中沙眼衣原体的比较。
J Clin Microbiol. 2004 Jul;42(7):3041-5. doi: 10.1128/JCM.42.7.3041-3045.2004.
4
Prevalence of chlamydial and gonococcal infections among young adults in the United States.美国年轻成年人中衣原体和淋病感染的患病率。
JAMA. 2004 May 12;291(18):2229-36. doi: 10.1001/jama.291.18.2229.
5
The effect of urine testing in evaluations of the sensitivity of the Gen-Probe Aptima Combo 2 assay on endocervical swabs for Chlamydia trachomatis and neisseria gonorrhoeae: the infected patient standard reduces sensitivity of single site evaluation.尿液检测对Gen-Probe Aptima Combo 2检测法检测沙眼衣原体和淋病奈瑟菌宫颈拭子敏感性评估的影响：感染患者标准降低了单部位评估的敏感性。
Sex Transm Dis. 2004 May;31(5):273-7. doi: 10.1097/01.olq.0000124611.73009.d5.
6
Vaginal swabs are appropriate specimens for diagnosis of genital tract infection with Chlamydia trachomatis.阴道拭子是诊断沙眼衣原体生殖道感染的合适标本。
J Clin Microbiol. 2003 Aug;41(8):3784-9. doi: 10.1128/JCM.41.8.3784-3789.2003.
7
Specimen processing and concentration of Chlamydia trachomatis added can influence false-negative rates in the LCx assay but not in the APTIMA Combo 2 assay when testing for inhibitors.在检测抑制剂时，添加沙眼衣原体的样本处理和浓缩会影响LCx检测中的假阴性率，但不会影响APTIMA Combo 2检测中的假阴性率。
J Clin Microbiol. 2003 Feb;41(2):778-82. doi: 10.1128/JCM.41.2.778-782.2003.
8
Chlamydia trachomatis diagnostics.沙眼衣原体诊断
Sex Transm Infect. 2002 Aug;78(4):232-4. doi: 10.1136/sti.78.4.232.
9
Replicate PCR testing and probit analysis for detection and quantitation of Chlamydia pneumoniae in clinical specimens.用于临床标本中肺炎衣原体检测和定量的重复聚合酶链反应（PCR）检测及概率分析。
J Clin Microbiol. 2001 May;39(5):1796-801. doi: 10.1128/JCM.39.5.1796-1801.2001.
10
Multicenter evaluation of the BDProbeTec ET System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs.BDProbeTec ET系统用于检测尿液标本、女性宫颈拭子和男性尿道拭子中沙眼衣原体和淋病奈瑟菌的多中心评估。
J Clin Microbiol. 2001 Mar;39(3):1008-16. doi: 10.1128/JCM.39.3.1008-1016.2001.
Zotero 插件