Attoumbré Jacques, Charlet Stéphane, Baltora-Rosset Sylvie, Hano Christophe, Raynaud-Le Grandic Sophie, Gillet Françoise, Bensaddek Lamine, Mesnard François, Fliniaux Marc-André
Biologie des plantes et contrôle des insectes ravageurs, EA 3900, Groupe de Phytotechnologie, Faculté de Pharmacie, 1 rue des Louvels, 80037, Amiens Cedex 1, France.
Plant Cell Rep. 2006 Aug;25(8):859-64. doi: 10.1007/s00299-006-0137-2. Epub 2006 Mar 8.
As flaxseed mainly accumulates lignans (secoisolariciresinol diglucoside and matairesinol), these compounds were barely or not detected in plant cell suspensions initiated from Linum usitatissimum. In contrast, these cell suspensions were shown to accumulate substantial amounts of a neolignan identified as dehydrodiconiferyl alcohol-4-beta-D: -glucoside (DCG) (up to 47.7 mg g(-1) DW). The formation of this pharmacologically active compound was evaluated as a function of cell growth and in relation to phytohormone balance of the culture media. After establishment of efficient culture conditions, production of DCG was investigated in immobilized plant cell suspensions initiated from plantlet roots of L. usitatissimum. The results indicate that immobilization enhances the DCG production up to 60.0 mg g(-1) DW but depresses the cell growth resulting in no improvement of the total DCG yield. Nevertheless, with immobilized cell suspensions, a release of DCG into the medium is observed allowing an easier recovery.
由于亚麻籽主要积累木脂素（开环异落叶松脂醇二葡萄糖苷和matairesinol），在由亚麻（Linum usitatissimum）起始的植物细胞悬浮液中几乎检测不到或未检测到这些化合物。相反，这些细胞悬浮液被证明积累了大量鉴定为脱氢松柏醇-4-β-D：-葡萄糖苷（DCG）的新木脂素（高达47.7 mg g(-1)干重）。评估了这种药理活性化合物的形成与细胞生长的关系以及与培养基植物激素平衡的关系。在建立高效培养条件后，研究了从亚麻幼苗根起始的固定化植物细胞悬浮液中DCG的产生。结果表明，固定化可将DCG产量提高到60.0 mg g(-1)干重，但会抑制细胞生长，导致DCG总产量没有提高。然而，对于固定化细胞悬浮液，观察到DCG释放到培养基中，便于回收。
