Weisburger J H, Madison R M, Ward J M, Viguera C, Weisburger E K
J Natl Cancer Inst. 1975 May;54(5):1185-8. doi: 10.1093/jnci/54.5.1185.
Administration of 40 ppm diethylnitrosamine (DENA) in the drinking water for 10 weeks to male Fischer rats led to hepatocellular carcinoma in 100 percent with metastasis to the lung in 40 percent, of the animals living for the full experimental period of 20 weeks. Concurrent feeding of phenobarbital and DENA for 10 weeks produced cancer of the liver in 77 percent of the animals, but only 9 percent had metastases in the lung. A brief regimen of DENA for 4 weeks, followed by 16 weeks of observation, induced cancer of the liver in only 13 percent of the rats. Administration of phenobarbital, begun 1 week after cessation of DENA intake and terminated at week 20, led to liver cancer in 64 percent of the rodents. Hydroxyurea had no effect on this enhancement. Treatment with a purified gamma fraction of antilymphocytic serum after the DENA did not influence the outcome. Thus phenobarbital given together with DENA reduced the severity of the carcinogenic process, but when it was given after the hepatocarcinogen, it increased the effect.
给雄性Fischer大鼠饮用含40 ppm二乙基亚硝胺（DENA）的水10周，在为期20周的完整实验期内存活的动物中，100%发生肝细胞癌，40%发生肺转移。同时给予苯巴比妥和DENA 10周，77%的动物发生肝癌，但只有9%有肺转移。DENA短期给药4周，随后观察16周，仅13%的大鼠诱发肝癌。在停止摄入DENA 1周后开始给予苯巴比妥并在第20周结束，64%的啮齿动物发生肝癌。羟基脲对此增强作用无影响。在给予DENA后用纯化的抗淋巴细胞血清γ组分治疗不影响结果。因此，与DENA同时给予苯巴比妥可降低致癌过程的严重程度，但在给予肝癌致癌物后给予苯巴比妥，则会增强其作用。
