Appl Environ Microbiol. 1995 Aug;61(8):2911-8. doi: 10.1128/aem.61.8.2911-2918.1995.
Five fungal species, Aspergillus versicolor, Penicillium commune, Cladosporium cladosporioides, Paecilomyces variotii, and Phialophora fastigiata, were cultivated on two media, malt extract agar and dichloran glycerol agar. Culture flasks provided with inlet and outlet tubes were used and purified, and humidified air was constantly led through the flasks. Air samples from the cultures were sorbed on Tenax GR and analyzed by thermal desorption-gas chromatography. The produced volatile metabolites were analyzed by mass spectrometry. Various hydrocarbons, alcohols, ketones, ethers, esters, sulfur-containing compounds, and terpenes were identified. The most commonly produced substances were 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, 3-methylfuran, and dimethyl disulfide. The production was highly dependent on both medium and species.
五种真菌物种,包括变色曲霉、普通青霉、枝孢霉、多形拟青霉和帚梗青霉,在两种培养基上进行了培养,即麦芽提取物琼脂和二氯甲烷甘油琼脂。使用带有进出口管的培养瓶进行培养,并保持空气的纯净和湿润,使纯化、加湿的空气不断通过培养瓶。从培养物中采集的空气样本被吸附在 Tenax GR 上,并通过热解吸-气相色谱进行分析。产生的挥发性代谢物通过质谱分析进行鉴定。鉴定出了各种烃类、醇类、酮类、醚类、酯类、含硫化合物和萜烯。最常产生的物质是 2-甲基-1-丙醇、2-甲基-1-丁醇、3-甲基-1-丁醇、3-甲基呋喃和二甲基二硫醚。其产生情况高度依赖于培养基和真菌种类。