Smits A, Kato M, Westermark B, Nistér M, Heldin C H, Funa K
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8159-63. doi: 10.1073/pnas.88.18.8159.
The different isoforms of PDGF bind with different affinities to two different receptor types. Previously, these receptors have been demonstrated mainly on mesenchymal and glial cells. We show here that PDGF beta-type receptors are present also on rat brain neurons in vivo as well as in vitro. Immunohistochemical analysis of a sequentially sectioned brain of a newborn rat revealed the presence of PDGF beta receptors in neurons throughout the central nervous system. These beta-receptor-bearing cells were shown to contain neurofilaments by double immunofluorescence staining. Furthermore, the PDGF-BB isoform bound to cells in a neuron-enriched culture from newborn rat brains and induced c-Fos protein in these cells. Continuous PDGF-BB treatment of primary rat brain cell cultures resulted in outgrowth of neurites and prolonged survival. These results indicate a neurotrophic effect of PDGF-BB.
血小板衍生生长因子（PDGF）的不同异构体以不同亲和力与两种不同的受体类型结合。此前，这些受体主要在间充质细胞和神经胶质细胞上得到证实。我们在此表明，PDGFβ型受体在体内外的大鼠脑神经元中也存在。对新生大鼠连续切片脑进行免疫组织化学分析发现，整个中枢神经系统的神经元中都存在PDGFβ受体。通过双重免疫荧光染色显示，这些带有β受体的细胞含有神经丝。此外，PDGF - BB异构体与新生大鼠脑富含神经元的培养物中的细胞结合，并在这些细胞中诱导c - Fos蛋白。对原代大鼠脑细胞培养物持续进行PDGF - BB处理导致神经突生长和存活期延长。这些结果表明PDGF - BB具有神经营养作用。
